Highly sensitive plasmonic nanorod hyperbolic metamaterial biosensor
Highly sensitive plasmonic nanorod hyperbolic metamaterial biosensor
复制标题
高灵敏等离子体纳米棒双曲超材料生物传感器
DOI：
10.1364/prj.444490
复制
发表时间：
2022-01-01
影响因子：
7.6
通讯作者：
Zhang, Jinyan
中科院分区：
文献类型：
--
作者：
Yan, Ruoqin;Wang, Tao;Zhang, Jinyan
基于纳米结构的等离子体传感是超灵敏无标记生物分子检测的一种强有力的分析工具，在临床诊断和生物医学研究领域具有巨大的潜力。在这里，我们报道了金纳米棒双曲异质材料的制备、表征和工作原理，以及超灵敏的体折射率和无标记生物分子检测。通过结合电子束光刻和纳米级电镀，我们展示了高度有序、高度可控的垂直纳米棒阵列的制备。通过棱镜耦合技术激发NHMM中的体等离子体激元-偏振子模式，并在微流体中集成该传感器，我们的器件的体灵敏度和优值系数分别达到了41600 nm/RIU和416RIU-1。通过理论和实验研究，揭示了这种高体灵敏度的物理机制。此外，通过对NHMM传感器表面进行生物功能化，可以实时监测稀释浓度下链霉亲和素的结合情况。我们测试了不同浓度的链霉亲和素，范围从200到5微克/毫升，NHMM生物传感器对5微克/毫升的链霉亲和素的检测显示出1 nm的波长漂移。通过拟合NHMM生物传感器的Hill方程，并考虑噪声水平(0.05 nm)作为检测限的最小波长漂移，估计NHMM生物传感器对链霉亲和素的检出限为0.14微克/毫升(2.4 nm)。作为直接对比，报道了在相同的实验条件下，使用常规金膜传感器时，链霉亲和素的波长漂移了20微克/毫升，波长移动了0.5 nm。所开发的等离子体NHMM传感器在高灵敏的本体溶液和生物分子检测方面显示出巨大的潜力，并为未来的自由标记生物传感应用提供了一条很有前途的途径。(C)2021年中国激光出版社
Plasmonic sensing based on nanostructures is a powerful analytical tool for ultrasensitive label-free biomolecule detection that holds great potential in the field of clinical diagnostics and biomedical research. Here, we report the fabrication, the characterization, and the principle of operation of gold nanorod hyperbolic metamaterials (NHMMs) along with ultrasensitive bulk refractive index and label-free biomolecular detection. By combining electron-beam lithography and nanoscale electroplating, we demonstrate the fabrication of a highly ordered, height-controllable, and vertical array of nanorods. By exciting the bulk plasmon-polariton mode in the NHMM using a prism-coupling technique and integrating the sensor in microfluidics, we demonstrate that the bulk sensitivity and figure of merit of our device could reach 41,600 nm/RIU and 416 RIU-1, respectively. The physical mechanism of this high bulk sensitivity is revealed through theoretical and experimental studies. Moreover, by bio-functionalizing the surface of the NHMM sensor, monitoring the binding of streptavidin at dilute concentrations is performed in real time. We test different concentrations of streptavidin ranging from 200 to 5 mu g/mL, and the NHMM biosensor exhibits a 1 nm wavelength shift for a 5 mu g/mL streptavidin detection. By fitting the Hill equation of the NHMM biosensor and taking into account the level of noise (0.05 nm) as the minimum wavelength shift of the detectable limit, the limit of detection of the NHMM biosensor to streptavidin can be estimated to be 0.14 mu g/mL (2.4 nm). As a direct comparison, a 0.5 nm wavelength shift for 20 mu g/mL of streptavidin is reported when using a conventional gold film sensor under identical experimental conditions. The developed plasmonic NHMM sensor shows tremendous potential for highly sensitive bulk solutions and biomolecule detection and provides a promising avenue for free-label biosensing applications in the future. (C) 2021 Chinese Laser Press