Hfq mutation confers increased cephalosporin resistance in Klebsiella pneumoniae
Hfq mutation confers increased cephalosporin resistance in Klebsiella pneumoniae
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Hfq 突变导致肺炎克雷伯菌头孢菌素耐药性增加
DOI：
10.2298/abs160126078l
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发表时间：
2017-03
影响因子：
0.8
通讯作者：
Chen Zeliang
中科院分区：
文献类型：
--
作者：
Li Xinran;Yang Mingjuan;Ke Yuehua;Liu Mingyuan;Wang Yufei;Liu Shiwei;Liu Bo;Chen Zeliang
肺炎克雷伯菌（K.肺炎杆菌（pneumoniae）是一种机会致病菌，由于其多重耐药性而引起了重大的公共卫生问题。Hfq是一种主要的RNA结合蛋白，是一种关键的转录后调节因子。该蛋白与多种病原菌的毒力和抗性密切相关。虽然hfq在K.尽管已经探索了肺炎克雷伯氏菌的毒力，但其对耐药性的影响在很大程度上仍然未知。本研究的目的是研究hfq在K. pneumoniae肺炎头孢菌素。构建hfq突变株，并研究其对头孢菌素的耐药性。hfq突变体表现出超过16倍的头孢菌素耐药性比野生型。时间-杀灭曲线分析表明，hfq突变株能在较高浓度的头孢菌素下存活。定量RT-PCR结果显示，在hfq突变体中，9种青霉素结合蛋白中有8种的表达水平下调，这些青霉素结合蛋白是头孢菌素的靶点。总之，与其在许多其他细菌中的作用相反，hfq参与K的负调节。通过下调青霉素结合蛋白的表达来降低肺炎克雷伯菌对头孢菌素的耐药性。DOI：10.2298/ABS 160126078 L投稿时间：2016 - 01 - 26修订时间：2016 - 03 - 20受理时间：2016 - 04 - 30网络发表时间：2016 - 09 - 14文章引用方式：Li X，Yang M，Ke Y，Liu M，Wang Y，Liu S，Liu B，Chen Z.肺炎克雷伯菌中的Hfq突变导致头孢菌素耐药性增加Arch Biol Sci. 2017;69（1）：61-9。
Klebsiella pneumoniae ( K. pneumoniae ), is an opportunistic pathogen raising significant public health concerns owing to its multi-drug resistance. Hfq, one of the main RNA-binding proteins, is a key post-transcriptional regulator. This protein is closely related to virulence and resistance in various pathogenic bacteria. Although the role of hfq in K. pneumoniae virulence has been explored, its influence on resistance remains largely unknown. The aim of this study was to investigate the role of hfq in the resistance of K. pneumoniae to cephalosporins. An hfq mutant was constructed, and its resistance to cephalosporins was investigated. The hfq mutant exhibited over 16-fold higher cephalosporin resistance than that exhibited by the wild type. Time-kill curve analysis showed that the hfq mutant could survive under higher concentrations of cephalosporins than the wild-type strain could. Quantitative RT-PCR showed that expression levels for 8 out of the 9 penicillin-binding proteins, which are the targets of cephalosporins, were downregulated in the hfq mutant. Taken together, contrary to its role in many other bacteria, hfq is involved in a negative regulation of K. pneumoniae resistance to cephalosporins by downregulating the expression of penicillin-binding proteins. DOI: 10.2298/ABS160126078L Received: January 26, 2016; Revised: March 20, 2016; Accepted: April 30, 2016; Published online: September 14, 2016 How to cite this article: Li X, Yang M, Ke Y, Liu M, Wang Y, Liu S, Liu B, Chen Z. Hfq mutation confers increased cephalosporin resistance in Klebsiella pneumoniae. Arch Biol Sci. 2017;69(1):61-9.
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DOI：
--
发表时间：
2011-03
期刊：
The Indian Journal of Medical Research
影响因子：
--
作者：
J. Vashist;Vishvanath Tiwari;R. Das;A. Kapil;M. Rajeswari
通讯作者：
J. Vashist;Vishvanath Tiwari;R. Das;A. Kapil;M. Rajeswari
影响因子：
3.6
作者：
Yanpeng Ding;B. M. Davis;M. Waldor
通讯作者：
Yanpeng Ding;B. M. Davis;M. Waldor
影响因子：
3.2
作者：
Christiansen, JK;Larsen, MH;Kallipolitis, BH
通讯作者：
Kallipolitis, BH
影响因子：
2.3
作者：
C. Banapurmath;S. Kallinath;S. Banapurmath;A. Kalliath;N. Kesaree
通讯作者：
C. Banapurmath;S. Kallinath;S. Banapurmath;A. Kalliath;N. Kesaree
RNA伴侣HFQ对于沙门氏菌伤寒的毒力至关重要。
影响因子：
3.6
作者：
Sittka A;Pfeiffer V;Tedin K;Vogel J
通讯作者：
Vogel J