Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn
Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn
复制标题
DOI：
10.1006/exer.1999.0794
复制
发表时间：
2000-03-01
影响因子：
3.4
通讯作者：
Song, CY
中科院分区：
文献类型：
--
作者：
Kim, JS;Kim, JC;Song, CY
羊膜（AM）含有基底膜成分和多种蛋白酶抑制剂。此外，当用作移植物时，AM的基底膜可以阻断对受损角膜表面的炎性损伤。因此，我们评估羊膜修补是否可以通过抑制蛋白水解损伤来促进愈合过程。通过应用直径为6.0 mm的圆形滤纸在兔的中央角膜上造成碱伤口。在1 N NaOH中浸泡30秒。伤后立即在角膜缘周围巩膜上进行羊膜修补。将115只家兔分为4组：（1）羊膜细胞侧向下覆盖至角膜缘周围巩膜（n = 26）;（2）羊膜基质侧向下覆盖至角膜缘周围巩膜（n = 19）;（3）锚定至穹窿（n = 29）;（4）未覆盖作为对照（n = 41）。术后3天取出AM。随访期间，测量每只眼角膜上皮缺损、角膜厚度和角膜混浊度。取部分角膜进行组织病理学研究、蛋白酶活性测定和酶谱分析。3个AM覆盖组角膜上皮愈合速度均快于对照组，角膜厚度均较对照组厚（P < 0.05）。覆盖组与锚定组之间无显著性差异（P > 0.05）。羊膜细胞侧向下组角膜混浊最轻，羊膜覆盖组的多形核白细胞（PMNs）浸润明显少于对照组。病理结果与酶谱结果相关，未覆盖组的蛋白酶活性明显高于AM覆盖组。急性碱烧伤后立即用AM作为临时补片进行干预，通过抑制蛋白酶活性和中性粒细胞浸润促进创面愈合。(C)北京大学出版社.
Amniotic membrane (AM) contains basement membrane components and various proteinase inhibitors. Furthermore, when used as a graft, the basement membrane of AM could block inflammatory insults to a damaged corneal surface. Thus, we evaluated whether amniotic membrane patching could promote the healing process by inhibiting proteolytic damage. Alkali wounds were inflicted on the central corneas of rabbits by applying a round filter paper, 6.0 mm in diameter. soaked in 1 N NaOH for 30 sec. Amniotic membrane patching was performed over the perilimbal sclera immediately after wounding. A total of 115 rabbits were divided into four groups: (1) immediately covered by AM with the amnion cell side down up to the perilimbal sclera (n = 26): (2) covered by AM with the stromal side down up to the perilimbal sclera (n = 19): (3) anchored to the fornix (n = 29); and (4) uncovered as a control (n = 41). AM was removed 3 days postoperatively. During follow-ups, epithelial defects, corneal thickness and its opacity of each eye were measured. Some corneas were removed for histopathologic studies and for proteinase activity ar:say and zymography. The epithelial healing was faster and the corneal thickness was thicker in all three AM-covered groups than in the control (P < 0.05). No significant difference was found between covered and anchored groups (P > 0.05). Corneal opacity was least in the amnion cell side down group, infiltration of polymorphonuclear leukocytes (PMNs) was much less in AM-covered groups than in the control. Pathological results were associated with zymographic findings, which revealed much higher proteinase activity in uncovered group than AM-covered groups. Immediate intervention for acute alkali burns with AM as a temporary patch promotes wound healing by inhibiting proteinase activity and PMNs infiltration. (C) 2000 Academic Press.