Development of a Species-Specific Coproantigen ELISA for Human Taenia solium Taeniasis
Development of a Species-Specific Coproantigen ELISA for Human Taenia solium Taeniasis
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DOI：
10.4269/ajtmh.2009.81.433
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发表时间：
2009-09-01
影响因子：
3.3
通讯作者：
Craig, Philip S.
中科院分区：
文献类型：
--
作者：
Guezala, Maria-Claudia;Rodriguez, Silvia;Craig, Philip S.
猪带绦虫引起人类神经囊尾蚴病，在后院养猪很常见的不发达国家流行。人类猪带绦虫病的粪便显微诊断方法不是很敏感，在光学显微镜下无法区分猪带绦虫和猪带绦虫卵。粪抗原 (CoAg) ELISA 方法非常灵敏，但目前仅对绦虫属 (Taenia) 具有特异性。本文介绍了一种高度物种特异性粪抗原 ELISA 测试的开发，用于检测猪带绦虫病。使用针对猪绦虫成虫全蠕虫体细胞提取物的兔 IgG 捕获抗体保持灵敏度，而通过利用针对猪绦虫成虫排泄-分泌 (ES) 抗原的酶缀合兔 IgG 来实现物种特异性。使用这种混合夹心 ELISA 测试了一组已知的阳性和阴性人类粪便样本。 ELISA 测试对猪绦虫携带者 (N = 28) 的特异性为 100%，敏感性为 96.4%，J 指数为 0.96。这种简单的 ELISA 结合了抗成虫体细胞抗体和抗成虫 ES 抗体，为人类猪带绦虫病提供了第一个潜在的物种特异性粪抗原测试。
Taenia solium causes human neurocysticercosis and is endemic in underdeveloped countries where backyard pig keeping is common. Microscopic fecal diagnostic methods for human T solium taeniasis are not very sensitive, and Taenia saginata and Taenia solium eggs are indistinguishable under the light microscope. Coproantigen (CoAg) ELISA methods are very sensitive, but currently only genus (Taenia) specific. This paper describes the development of a highly species-specific coproantigen ELISA test to detect T. solium intestinal taeniasis. Sensitivity was maintained using a capture antibody of rabbit IgG against T. solium adult whole worm somatic extract, whereas species specificity was achieved by utilization of an enzyme-conjugated rabbit IgG against T. solium adult excretory-secretory (ES) antigen. A known panel of positive and negative human fecal samples was tested with this hybrid sandwich ELISA. The ELISA test gave 100% specificity and 96.4% sensitivity for T. solium tapeworm carriers (N = 28), with a J index of 0.96. This simple ELISA incorporating anti-adult somatic and anti-adult ES antibodies provides the first potentially species-specific coproantigen test for human T. solium taeniasis.