DISTRIBUTION OF GABA-LIKE IMMUNOREACTIVITY IN THE PIGEON BRAIN
DISTRIBUTION OF GABA-LIKE IMMUNOREACTIVITY IN THE PIGEON BRAIN
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DOI：
10.1016/0306-4522(88)90047-4
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发表时间：
1988-06-01
期刊：
影响因子：
3.3
通讯作者：
STREIT, P
中科院分区：
文献类型：
--
作者：
DOMENICI, L;WALDVOGEL, HJ;STREIT, P
用单抗研究了戊二醛固定的鸽脑内GABA样免疫反应阳性物质的分布。GABA免疫反应阳性反应见于不同大小的神经纤维束、神经纤维束和神经纤维束。某些染色模式表明，鸽脑中存在几个GABA能投射系统。事实上，顶盖下核和腹后核高密度的免疫染色的包被和低密度的标记的终末样元件是显著的模式，而在峡部-视神经核内观察到缺乏GABA样免疫反应和免疫反应点的聚集等。在视顶盖，在第III(10)层可见具有放射状突起的染色细胞体，在第IID(5)层可见具有水平定向突起的染色细胞体，这使人想起先前在顶盖注射氚-GABA后所获得的放射自显影标记模式。在小脑，GABA样免疫反应涉及除颗粒细胞以外的所有类型的神经元。浦肯野细胞呈区域性不同的免疫染色强度。此外，在Folium X中没有观察到染色的篮状分子。虽然到目前为止还没有证据表明GABA在大多数结构中的功能，但目前的结果表明这种神经递质在鸽子大脑中发挥着重要作用。
The distribution of GABA-like immunoreactivity in glutaraldehyde-fixed pigeon brains was studied by means of a monoclonal antibody. GABA-like immunoreactivity was observed in neuronal perikarya of different sizes as well as in neuropil and in certain fiber tracts. Certain staining patterns indicated the existence of several GABAergic projection systems in the pigeon brain. Indeed, a high density of immunostained perikarya and a low density of labeled terminal-like elements was the prominent pattern in the nuclei subpretectalis and posteroventralis, while an absence of perikaryal GABA-like immunoreactivity and accumulations of immunoreactive dots were observed in the isthmo-optic nucleus, amongst others. In the optic tectum, stained cell bodies with radially oriented processes in layer IIi (10) and with horizontally oriented processes in layer IId (5) were seen and were reminiscent of autoradiographic labeling patterns obtained previously following tectal injection of tritiated GABA. In the cerebellum, GABA-like immunoreactivity involved all types of neurons with the exception of granule cells. Purkinje cells showed regionally different intensities of immunostaining. In addition, in folium X no stained basket-like elements were observed. Although there is no evidence as yet about the function of GABA in most of the structures, the present results indicate an important role for this neurotransmitter in the pigeon brain.