Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury.
Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury.
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DOI：
10.3892/mmr.2018.8918
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发表时间：
2018-06
影响因子：
3.4
通讯作者：
Shujie Zhao;Wei Zhou;Jian Chen-;Yongjun Luo;G. Yin
中科院分区：
文献类型：
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作者：
Shujie Zhao;Wei Zhou;Jian Chen-;Yongjun Luo;G. Yin
脊髓损伤(SCI)是致残的原因之一。本研究旨在探讨创伤性脊髓损伤的分子机制。登录号：转录组数据。GSE5296，包括96个芯片，从基因表达总括数据库下载。对原始数据进行归一化，并鉴定差异表达基因(Deg)。此外，还进行了京都百科全书的基因和基因组途径以及上调和下调的DEGS的基因本体论富集性分析。此外，还构建了蛋白质-蛋白质相互作用网络，并确定了不同基因的表达模式。与假手术组相比，伤后0.5、4、24、72、7、28天分别检测到374、707、1,322、1,475、1,724和1,342°。在伤后24、72小时和7天，上调的DEGS的“炎症反应”和“免疫过程”明显增强。下调的DEG主要集中在神经功能相关的通路和类固醇生物合成过程中。蛋白质-蛋白质相互作用网络分析也得到了类似的结果。趋势图进一步表明，炎症和神经元功能发生了时间和部位特异性的改变。本研究为创伤性脊髓损伤的分子机制研究提供了新的思路，有助于今后的脊髓损伤研究和治疗的发展。
Spinal cord injury (SCI) is a cause of disability. The present study aimed to investigate the molecular mechanisms involved in traumatic SCI. Transcriptome data under accession no. GSE5296, including 96 chips, were downloaded from the Gene Expression Omnibus database. The raw data were normalized and differentially expressed genes (DEGs) were identified. Furthermore, Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology enrichment analysis of up‑ and downregulated DEGs was performed. Additionally, a protein‑protein interaction network was constructed and the expression patterns of different genes were determined. Compared with sham samples, there were 374, 707, 1,322, 1,475, 1,724 and 1,342 DEGs identified at 0.5, 4, 24 and 72 h, and 7 and 28 days post‑injury, respectively. At 24 and 72 h, and 7 days following injury, the upregulated DEGs were markedly enriched in 'inflammatory response' and 'immune process'. Downregulated DEGs were predominantly enriched in neuronal function‑associated pathways and 'steroid biosynthesis' process. Protein‑protein interaction network analysis demonstrated similar results. Trend charts further demonstrated that the inflammatory and neuronal functions were altered in a temporal and site‑specific manner. The present study provided an insight into the molecular mechanisms underlying traumatic SCI, which may benefit future SCI research and aid in therapy development.