Temperature-dependent RNA editing in octopus extensively recodes the neural proteome
Temperature-dependent RNA editing in octopus extensively recodes the neural proteome
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DOI：
10.1016/j.cell.2023.05.004
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发表时间：
2023-06-08
期刊：
影响因子：
64.5
通讯作者：
Rosenthal, Joshua J. C.
中科院分区：
文献类型：
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作者：
Birk, Matthew A.;Liscovitch-Brauer, Noa;Rosenthal, Joshua J. C.
在变温动物中，温度变化挑战生理功能的整合。在复杂的神经系统的行为复杂的鞘翅目头足类动物，这些问题是实质性的。通过腺苷脱氨进行RNA编辑是环境适应的良好定位机制。我们报告，章鱼bimaculoides的神经蛋白质组经历了大规模的重组，通过RNA编辑后的温度挑战。超过13，000个密码子受到影响，其中许多改变了对神经过程至关重要的蛋白质。对于两个高度温度敏感的例子，重新编码调节蛋白质功能。突触结合蛋白是钙依赖性神经递质释放的关键成分，晶体结构和支持实验表明，编辑改变了钙结合。对于驱动蛋白-1，驱动轴突运输的马达蛋白，编辑调节微管下的运输速度。对野生捕获标本的季节性采样表明，在野外也会发生温度依赖性编辑。这些数据表明，A-to-I编辑调整了章鱼和其他海鞘的神经生理功能，以响应温度。
In poikilotherms, temperature changes challenge the integration of physiological function. Within the com-plex nervous systems of the behaviorally sophisticated coleoid cephalopods, these problems are substan-tial. RNA editing by adenosine deamination is a well-positioned mechanism for environmental acclimation. We report that the neural proteome of Octopus bimaculoides undergoes massive reconfigurations via RNA editing following a temperature challenge. Over 13,000 codons are affected, and many alter proteins that are vital for neural processes. For two highly temperature-sensitive examples, recoding tunes protein function. For synaptotagmin, a key component of Ca2+-dependent neurotransmitter release, crystal struc-tures and supporting experiments show that editing alters Ca2+ binding. For kinesin-1, a motor protein driving axonal transport, editing regulates transport velocity down microtubules. Seasonal sampling of wild-caught specimens indicates that temperature-dependent editing occurs in the field as well. These data show that A-to-I editing tunes neurophysiological function in response to temperature in octopus and most likely other coleoids.