Enterococcus faecium WEFA23 from infants lessens high-fat-diet-induced hyperlipidemia via cholesterol 7-alpha-hydroxylase gene by altering the composition of gut microbiota in rats
Enterococcus faecium WEFA23 from infants lessens high-fat-diet-induced hyperlipidemia via cholesterol 7-alpha-hydroxylase gene by altering the composition of gut microbiota in rats
复制标题
来自婴儿的屎肠球菌 WEFA23 通过改变大鼠肠道微生物群的组成，通过胆固醇 7-α-羟化酶基因减轻高脂饮食诱导的高脂血症
DOI：
10.3168/jds.2017-13713
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发表时间：
2018-09-01
影响因子：
3.5
通讯作者：
Wei, Hua
中科院分区：
文献类型：
--
作者：
Huang, Fuqing;Zhang, Fen;Wei, Hua
屎肠球菌WEFA 23是来自中国婴儿的潜在益生菌菌株，具有降低胆固醇水平的能力。目的探讨E.在体内实验中，我们检测了与胆固醇代谢相关的基因转录、粪便中胆汁酸的组成、肝脏中三乙胺N-氧化物（TMAO）的合成以及大鼠肠道菌群的组成。我们发现E. faecium WEFA 23通过促进胆固醇排泄、上调与胆固醇分解和转运相关的基因转录水平、下调与胆固醇合成相关的基因来增强胆汁酸的合成。此外，E. faecium WEFA 23不仅下调法尼醇X受体、成纤维细胞生长因子15和黄素单加氧酶3的转录水平，而且降低TMAO的产生，随后增加CYP 7A 1的转录水平。经口给药35 d，E. faecium WEFA 23改善了高脂饮食大鼠的肠道微生物群多样性。其中，拟杆菌门与厚壁菌门的比例和Rikenellaceae的丰度增加，而韦荣氏球菌科的数量减少。这些结果表明，E.屎肠WEFA 23可能与肠道微生物群的变化有关。我们的发现为E.屎肠球菌，并揭示了肠球菌属。可能会降低氧化三甲胺的含量
Enterococcus faecium WEFA23 is a potential probiotic strain from Chinese infants with the ability to decrease cholesterol levels. Aiming to explore the mechanism of E. faecium WEFA23 in lowering cholesterol in vivo, we examined the gene transcriptions related to cholesterol metabolism, the composition of bile acids in feces, the synthesis of trirnethylamine N-oxide (TMAO) in liver, and the composition of the gut microbiota of rats. We found that E. faecium WEFA23 enhanced the synthesis of bile acids by promoting cholesterol excretion, upregulating the genes transcript level relevant to cholesterol decomposition and transportation, and downregulating the genes involved in cholesterol synthesis. In addition, E. faecium WEFA23 not only downregulated the transcript levels of farnesoid X receptor and fibroblast growth factor 15 as well as flavin-containing mono-oxygenase 3, but also decreased the TMAO production followed by increasing the CYP7A1 transcript level. Furthermore, when orally administered to rats for 35 d, E. faecium WEFA23 improved the gut microbiota diversity of rats fed a high-fat diet. Therein, the ratio of Bacteroidetes to Firmicutes and the abundance of Rikenellaceae increased, whereas the number of Veillonellaceae decreased. These results suggest that reduction of cholesterol level by E. faecium WEFA23 might be related to the changes in the gut microbiota. Our finding provides important information on lowering cholesterol by E. faecium and reveals that Enterococcus spp. might have the potential to decrease the TMAO level.