Effect of ethanol on gastric mucus glycoprotein synthesis, translocation, transport, glycosylation, and secretion.
Effect of ethanol on gastric mucus glycoprotein synthesis, translocation, transport, glycosylation, and secretion.
复制标题
乙醇对胃粘液糖蛋白合成、易位、运输、糖基化和分泌的影响。
DOI：
10.1111/j.1530-0277.1997.tb03785.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Slomiany,BL
中科院分区：
文献类型：
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作者：
Slomiany,A;Morita,M;Sano,S;Piotrowski,J;Skrodzka,D;Slomiany,BL
研究了乙醇对大鼠胃粘膜细胞粘液糖蛋白合成、胞内修饰、转运、糖基化和分泌的影响。预孵育的体外翻译混合物含有胃粘液细胞mRNA的60分钟，0至120 mM乙醇引起的粘液糖蛋白载脂蛋白肽的合成减少高达40%。翻译的减少具有时间和乙醇浓度依赖性。60分钟后，在30、60和120 mM乙醇存在下的翻译分别降低至83.3 ± 2.3%、75.5 ± 0.4%和63.6 ± 2.6%。与内质网微粒体进行的实验，预孵育与乙醇，并在研究中使用的无粘蛋白的共翻译易位显示减少了20%的粘液糖蛋白载脂蛋白肽转移到内质网微粒体的内腔。在乙醇的存在下，粘液糖蛋白载脂肽在高尔基体的加工也受到抑制。在最初的30分钟的孵育与0至120 mM乙醇，糖基化似乎进行在样品中有和没有乙醇以相同的速率。然而，在连续30分钟的孵育过程中，糖基化在60 mM乙醇的存在下下降了30至35%，并与120 mM乙醇被完全抑制。测量乙醇对粘液糖蛋白从细胞内储存中排出的影响表明，平均而言，暴露于乙醇液体饮食8周的大鼠胃粘膜的分泌量减少了77%或更多，并且糖蛋白对胃上皮的粘附减弱。结果表明，乙醇抑制胃粘液糖蛋白的合成，运输和加工，并在不同的细胞内隔室发生的过程有助于添加剂的方式，并反映在一个急剧减少的粘液糖蛋白的胃上皮表面的交付。
The effect of ethanol on mucus glycoprotein synthesis, intracellular modification, transport, glycosylation, and secretion was studied in rat gastric mucous cells. Preincubation of the in vitro translation mixture containing gastric mucous cells mRNA for 60 min with 0 to 120 mM ethanol caused a decrease in the synthesis of mucus glycoprotein apopeptide by up to 40%. The reduction in translation was time‐ and ethanol concentration‐dependent. After 60 min, translation in the presence of 30, 60, and 120 mM ethanol decreased to 83.3 ± 2.3%, 75.5 ± 0.4%, and 63.6 ± 2.6%, respectively. The experiments conducted with endoplasmic reticulum microsomes, preincubated with ethanol, and used in the studies of cotranslational translocation of the apomucin showed a 20% decrease in the transfer of mucus glycoprotein apopeptide to the lumen of endoplasmic reticulum microsomes. In the presence of ethanol, processing of mucus glycoprotein apopeptide in Golgi was also inhibited. During the initial 30 min of incubation with 0 to 120 m M ethanol, glycosylation seemed to proceed at the same rate in the samples with and without ethanol. However, during consecutive 30 min of incubation, glycosylation in the presence of 60 mM ethanol decreased by 30 to 35%, and with 120 m M ethanol was completely inhibited. Measurements of the effect of ethanol on the discharge of mucus glycoprotein from the intracellular stores revealed that, on average, the secretory output of the rat gastric mucosa exposed to ethanol liquid diet for 8 weeks decreased by 77% or more, and adherence of the glycoprotein to the gastric epithelium was weakened. Results indicate that ethanol inhibits synthesis, transport, and processing of gastric mucus glycoprotein, and that the processes taking place in different intracellular compartments contribute in the additive fashion and, are reflected in a dramatic decrease in the delivery of mucus glycoprotein to the gastric epithelial surfaces.