Changes in skeletal muscle proteolytic gene expression after prophylactic supplementation of EGCG and NAC and eccentric damage
Changes in skeletal muscle proteolytic gene expression after prophylactic supplementation of EGCG and NAC and eccentric damage
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DOI：
10.1016/j.fct.2013.01.026
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发表时间：
2013-11-01
影响因子：
4.3
通讯作者：
Willoughby, Darryn S.
中科院分区：
文献类型：
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作者：
Kerksick, Chad M.;Roberts, Michael D.;Willoughby, Darryn S.
目的：研究了预防性补充N-乙酰半胱氨酸（NAC）和表没食子儿茶素没食子酸酯（EGCG）对不习惯的离心肌肉收缩后肌内蛋白水解基因表达的影响。（平均值+/- SD：20.0 ± 1.8岁，175 ± 7.1 cm，76.1 ± 16.9 kg），每日摄入1800 mg NAC或1800 mg EGCG（98%总多酚、80%总儿茶素和50%EGCG），或1000 mg葡甘露聚糖安慰剂（PLA），以双盲、预防性方式持续14天。然后，受试者完成一个不习惯的离心运动回合（100重复在30度s（-1））使用占主导地位的膝盖伸肌。在基线和运动后6小时和24小时从股外侧肌收集骨骼肌活检。蛋白水解基因的表达[即，肌肉环指蛋白1（MuRF 1）、atrogin-1、α-型20 S 20 S亚基C2（HC 2）、α-型20 S亚基C3（HC 3）、泛素蛋白连接酶3B（UBE 3B）、mu-钙蛋白酶和m-钙蛋白酶]使用实时RT-PCR定量。结果：对于任何atrogenes或钙蛋白酶的表达，在组之间没有检测到显著的组x时间相互作用（p > 0.05）。时间确定的MuRF增加的显著主要效应1（6 h：5.3 +/- 10.8倍; p = 0.046），UBE 3B（6 h：5.3 +/- 7.7倍; p = 0.006; 24 h：3.3 +/- 4.5倍; p = 0.005），m-钙蛋白酶表达（6小时：2.7 +/- 4.4倍; p = 0.045）。增加HC 2中的接近显著性（6 h：1.9 +/- 2.4倍; p = 0.079; 24 h：1.6 +/- 1.9倍; p = 0.084）和m-钙蛋白酶表达（24 h; 1.8 +/- 2.3倍; p = 0.084）。预防性补充NAC和表没食子儿茶素没食子酸酯没有影响离心运动后骨骼肌蛋白水解基因表达的急性变化。肌肉离心收缩使MuRF 1和UBE 3B表达升高，而m-钙蛋白酶和HC 2 mRNA表达有升高的趋势。(C)2013爱思唯尔有限公司保留所有权利。
Purpose: The impact of prophylactic supplementation of N-acetyl-cysteine (NAC) and epigallocatechin gallate (EGCG) on intramuscular expression of proteolytic genes after unaccustomed eccentric muscle contractions was investigated.Methods: Thirty apparently healthy males (mean +/- SD: 20.0 +/- 1.8 years, 175 +/- 7.1 cm, 76.1 +/- 16.9 kg) ingested daily either 1800 mg of NAC or 1800 mg of EGCG (98% total polyphenols, 80% total catechins, and 50% EGCG), or 1000 mg of a glucomannan placebo (PLA) in a double blind, prophylactic fashion for 14 days. Subjects then completed an unaccustomed eccentric exercise bout (100 repetitions at 30 degrees s(-1)) using the dominant knee extensors. Skeletal muscle biopsies were collected from the vastus lateralis at baseline and both 6 and 24 h after exercise. The expression of proteolytic genes [i.e., muscle ring-finger 1 (MuRF1), atrogin-1, alpha-type 20S 20S subunit C2 (HC2), alpha-type 20S subunit C3 (HC3), ubiquitin protein ligase 3B (UBE3B), mu-calpain, and m-calpain] was quantified using real-time RT-PCR. Separate 3 x 3 (group x time) repeated measures ANO-VAs were used to analyze changes in gene expression over time between groups.Results: No significant group x time interactions were detected between groups for the expression of any of the atrogenes or calpains (p > 0.05). Significant main effects for time identified increases in MuRF1 (6 h: 5.3 +/- 10.8 fold; p = 0.046), UBE3B (6 h:5.3 +/- 7.7 fold; p = 0.006; 24 h: 3.3 +/- 4.5 fold; p = 0.005), and m-calpain expression (6 h: 2.7 +/- 4.4 fold; p = 0.045) in all participants following exercise. Increases approached significance in HC2 (6 h: 1.9 +/- 2.4 fold; p = 0.079; 24 h: 1.6 +/- 1.9 fold; p = 0.084) and m-calpain expression (24 h; 1.8 +/- 2.3 fold; p = 0.084) following exercise.Conclusions: Prophylactic supplementation of NAC and EGCG did not impact acute changes in skeletal muscle proteolytic gene expression following eccentric exercise. Eccentric muscle contractions elevated MuRF1 and UBE3B, while m-calpain and HC2 mRNA tended to increase. (C) 2013 Elsevier Ltd. All rights reserved.