From the genome to the proteome:: Uncovering peptides in the Apis brain
From the genome to the proteome:: Uncovering peptides in the Apis brain
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DOI：
10.1126/science.1124128
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发表时间：
2006-10-27
期刊：
影响因子：
56.9
通讯作者：
Sweedler, Jonathan V.
中科院分区：
文献类型：
--
作者：
Hummon, Amanda B.;Richmond, Timothy A.;Sweedler, Jonathan V.
神经肽是通过影响几乎每个神经元回路的活性来调节动物行为的关键脑肽，由于广泛的翻译后加工，从新生基因组序列直接预测神经肽本质上是困难的。生物信息学和蛋白质组学的结合允许从未注释的基因组中发现神经肽。在意大利蜜蜂基因组中，我们已经推断出200多个神经肽，并已确认100肽的序列。该研究为进一步研究蜜蜂神经肽奠定了基础，共鉴定了36个基因，其中33个是以前未报道的。
Neuropeptides, critical brain peptides that modulate animal behavior by affecting the activity of almost every neuronal circuit, are inherently difficult to predict directly from a nascent genome sequence because of extensive posttranslational processing. The combination of bioinformatics and proteomics allows unprecedented neuropeptide discovery from an unannotated genome. Within the Apis mellifera genome, we have inferred more than 200 neuropeptides and have confirmed the sequences of 100 peptides. This study lays the groundwork for future molecular studies of Apis neuropeptides with the identification of 36 genes, 33 of which were previously unreported.