PRESENCE OF VIRAL-ANTIGENS AND ANTIBODY IN THE TRACHEA OF CHICKENS INFECTED WITH AVIAN INFECTIOUS-BRONCHITIS VIRUS
PRESENCE OF VIRAL-ANTIGENS AND ANTIBODY IN THE TRACHEA OF CHICKENS INFECTED WITH AVIAN INFECTIOUS-BRONCHITIS VIRUS
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DOI：
10.1080/03079458308436175
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发表时间：
1983-01-01
期刊：
影响因子：
2.8
通讯作者：
CAVANAGH, D
中科院分区：
文献类型：
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作者：
HAWKES, RA;DARBYSHIRE, JH;CAVANAGH, D
用鸡传染性支气管炎病毒（M_（41）株）感染鸡后44天，用免疫荧光法在气管粘膜上皮基底层中检测到病毒抗原。酶联免疫吸附试验（ELISA）第一次检测到病毒特异性抗体在气管冲洗感染后7天，在10天，这些抗体达到的水平，并保持至少44天，虽然有相当大的差异鸡。中和试验直到第20天才检测到抗体;滴度在2.2-3.2 log 2范围内，在第27天达到最大值。ELISA仅在第7天检测到抗病毒伊加气管内冲洗液;在含有抗病毒抗体的所有样品中均检测到IgG。ELISA法检测血清抗病毒抗体在第7天，并在第10天达到峰值滴度，并保持。血清中和抗体在第10天首次检测到，并在第24天增加至峰值滴度。ELISA和中和试验的结果之间的差异的影响进行了讨论。
Following infection of chickens with infectious bronchitis virus (strain M41) viral antigens were detected by immunofluorescence in the basal layer of the tracheal mucosal epithelium for 44 days. The enzyme-linked immunosorbent assay (ELISA) 1st detected virus-specific antibody in tracheal washes 7 days after infection; at 10 days these antibodies reached a level that was maintained for at least 44 days, although there was considerable variation between chickens. A neutralization test did not detect antibody until day 20; titers were in the range 2.2-3.2 log2, reaching a maximum at day 27. ELISA detected anti-viral IgA intracheal washes only on day 7; IgG was detected in all samples containing anti-viral antibody. Serum anti-viral antibodies were detected by ELISA at 7 days and reached peak titers at 10 days, which were maintained. Serum neutralizing antibodies were first detected at 10 days and increased to peak titers on day 24. The implications of the differences between the results of ELISA and neutralization tests are discussed.