Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout.
Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout.
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DOI：
10.1186/1477-7827-8-34
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发表时间：
2010-04-12
期刊：
影响因子：
--
通讯作者：
Planas JV
中科院分区：
文献类型：
--
作者：
Crespo D;Bonnet E;Roher N;MacKenzie SA;Krasnov A;Goetz FW;Bobe J;Planas JV
免疫-内分泌相互作用与硬骨鱼类卵巢功能调节的相关性实际上尚未探索。作为感染期间先天免疫应答的一部分，产生许多细胞因子如肿瘤坏死因子α（TNF α）和其他免疫因子，并作用于生殖系统。然而，TNF α也是哺乳动物排卵过程中的重要生理参与者。在本研究中，我们首次研究了TNF α在体外对硬骨鱼，褐鳟鱼（萨尔莫trutta）排卵前卵泡的影响。为了确定卵巢中TNF α表达的体内调节，用盐水或细菌脂多糖（LPS）腹膜内注射排卵前的溪鳟（Salvelinus fontinalis）。在控制和重组鳟鱼TNF α（rtTNF α）处理的褐鳟鱼颗粒细胞，我们研究了通过流式细胞术分析和碘化丙啶（PI）染色细胞活力的凋亡百分比。此外，我们确定了在体外的影响，rtTNF α卵泡收缩和睾酮的生产在排卵前褐鳟鱼卵巢卵泡。此外，我们通过微阵列和实时PCR（qPCR）分析了对照组和rtTNF α治疗组卵巢组织的基因表达谱。体内给予LPS可显著诱导卵巢TNF α的表达。用rtTNF α治疗诱导颗粒细胞凋亡，降低颗粒细胞活力，并刺激已知参与鳟鱼正常排卵过程的基因表达。此外，rtTNF α导致卵泡收缩和睾酮产生显著增加。此外，使用鲑鱼特异性微阵列平台（SFA2.0免疫芯片），我们观察到rtTNF α诱导已知参与炎症、蛋白水解和组织重塑的基因的表达。此外，激肽释放酶、TOP-2、丝氨酸蛋白酶23和ADAM 22的表达在rtTNF α存在下孵育的卵泡中增加，这些基因被假定参与鳟鱼排卵期间的蛋白水解和组织重塑过程。鉴于这些结果，我们建议，TNF α可能有一个重要的作用，在生物力学的卵泡弱化，卵巢破裂和卵母细胞排出在鳟鱼排卵过程中，主要是通过刺激卵泡细胞凋亡和参与卵泡壁蛋白水解和收缩的基因的表达。
The relevance of immune-endocrine interactions to the regulation of ovarian function in teleosts is virtually unexplored. As part of the innate immune response during infection, a number of cytokines such as tumor necrosis factor alpha (TNF alpha) and other immune factors, are produced and act on the reproductive system. However, TNF alpha is also an important physiological player in the ovulatory process in mammals. In the present study, we have examined for the first time the effects of TNF alpha in vitro in preovulatory ovarian follicles of a teleost fish, the brown trout (Salmo trutta). To determine the in vivo regulation of TNF alpha expression in the ovary, preovulatory brook trout (Salvelinus fontinalis) were injected intraperitoneally with either saline or bacterial lipopolysaccharide (LPS). In control and recombinant trout TNF alpha (rtTNF alpha)-treated brown trout granulosa cells, we examined the percentage of apoptosis by flow cytometry analysis and cell viability by propidium iodide (PI) staining. Furthermore, we determined the in vitro effects of rtTNF alpha on follicle contraction and testosterone production in preovulatory brown trout ovarian follicles. In addition, we analyzed the gene expression profiles of control and rtTNF alpha-treated ovarian tissue by microarray and real-time PCR (qPCR) analyses. LPS administration in vivo causes a significant induction of the ovarian expression of TNF alpha. Treatment with rtTNF alpha induces granulosa cell apoptosis, decreases granulosa cell viability and stimulates the expression of genes known to be involved in the normal ovulatory process in trout. In addition, rtTNF alpha causes a significant increase in follicle contraction and testosterone production. Also, using a salmonid-specific microarray platform (SFA2.0 immunochip) we observed that rtTNF alpha induces the expression of genes known to be involved in inflammation, proteolysis and tissue remodeling. Furthermore, the expression of kallikrein, TOP-2, serine protease 23 and ADAM 22, genes that have been postulated to be involved in proteolytic and tissue remodeling processes during ovulation in trout, increases in follicles incubated in the presence of rtTNF alpha. In view of these results, we propose that TNF alpha could have an important role in the biomechanics of follicle weakening, ovarian rupture and oocyte expulsion during ovulation in trout, primarily through its stimulation of follicular cell apoptosis and the expression of genes involved in follicle wall proteolysis and contraction.
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影响因子：
3.6
作者：
Flaws, JA;Hirshfield, AN;Furth, PA
通讯作者：
Furth, PA
影响因子：
3.6
作者：
BERNDTSON, AK;GOETZ, FW
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3.6
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影响因子：
2.7
作者：
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影响因子：
3.6
作者：
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通讯作者：
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