Effects of aging and dietary restriction on ubiquitination, sumoylation, and the proteasome in the spleen.
Effects of aging and dietary restriction on ubiquitination, sumoylation, and the proteasome in the spleen.
复制标题
衰老和饮食限制对脾脏中泛素化、苏酰化和蛋白酶体的影响。
DOI：
10.1016/j.febslet.2007.10.054
复制
发表时间：
2007
期刊：
影响因子：
3.5
通讯作者：
Keller,JeffreyN
中科院分区：
文献类型：
--
作者：
Zhang,Le;Li,Feng;Dimayuga,Edgardo;Craddock,Jeffrey;Keller,JeffreyN
在本研究中，我们首次证明衰老会增加脾脏中泛素化蛋白的水平，而饮食限制（DR）可显着减少这些与年龄相关的泛素化蛋白的增加。脾脏中的苏酰化蛋白、蛋白酶体亚基和蛋白酶体生物发生必需的蛋白质 (POMP1) 也随着年龄的增长而增加，但不受 DR 的显着影响。老年脾脏中胰凝乳蛋白酶样蛋白酶体活性升高，并且 DR 没有显着改变。这些数据首次证明了衰老和 DR 对脾脏中泛素化、苏酰化和蛋白酶体的多重影响。
In the present study, we demonstrate for the first time that aging increases the levels of ubiquitinated protein in the spleen, and that dietary restriction (DR) significantly reduces these age-related increases in ubiquitinated protein. Sumoylated protein, proteasome subunits, and a protein essential for proteasome biogenesis (POMP1) were also increased with age in the spleen but were not significantly affected by DR. Chymotrypsin-like proteasome activity was elevated in the aged spleen, and was not significantly altered by DR. Together, these data demonstrate for the first time the multiple effects of aging and DR on ubiquitination, sumoylation, and the proteasome in the spleen.