A Peristaltic Pump and Filter-Based Method for Aqueous Microplastic Sampling and Analysis
A Peristaltic Pump and Filter-Based Method for Aqueous Microplastic Sampling and Analysis
复制标题
DOI：
10.1021/acsestwater.1c00270
复制
发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Z. Harrold;M. Arienzo;Meghan Collins;Julia M. Davidson;Xuelian Bai;Suja Sukumaran;J. Umek
中科院分区：
文献类型：
--
作者：
Z. Harrold;M. Arienzo;Meghan Collins;Julia M. Davidson;Xuelian Bai;Suja Sukumaran;J. Umek
由于微塑料浓度、形状和密度的变化以及污染的可能性，对水生环境中的微塑料浓度进行采样本身就很困难。我们对一种微塑料采样方法进行了评估，该方法使用蠕动泵将水泵入一系列串联不锈钢网过滤器。过滤后，使用先前公布的方法对不锈钢过滤器进行处理，以分离微塑料，并针对不锈钢网过滤器进行调整。使用透射模式下的微傅里叶变换红外 (μFTIR) 光谱来识别微塑料。该方法在实验室中使用标准聚乙烯珠进行了测试，并应用于内华达州拉斯维加斯清洗站的两个样本点。结果表明，经过蠕动泵和实验室步骤后，70% 的聚乙烯珠被回收，空白污染最小。蠕动泵采样方法的优点是 (1) 支持一系列样品体积，(2) 减少样品处理，(3) 减少污染的可能性，(4) 提供采样位置的灵活性，以及 (5) 支持各种过滤器类型。使用不锈钢网过滤器可以实现 (1) 简化和直接从现场到实验室的样品处理，(2) 对安装在过滤器上的微塑料进行 μFTIR 透射模式分析，以及 (3) 降低过滤器和样品处理成本。
Sampling the aquatic environment for microplastic concentration is inherently difficult because of variations in microplastic concentration, shape, and density and the potential for contamination. We present an assessment of a method for microplastic sampling that uses a peristaltic pump to pump water through a series of in-line stainless-steel mesh filters. Following filtration, the stainless-steel filters were treated using previously published methods to isolate microplastics, adjusted for the stainless-steel mesh filters. Microplastics were identified using micro-Fourier transform infrared (μFTIR) spectroscopy in transmission mode. This method was tested in the laboratory using standard polyethylene beads and was applied to two sample sites at the Las Vegas Wash in Nevada. The results showed that 70% of the polyethylene beads were recovered after the peristaltic pump and laboratory steps with minimal blank contamination. The advantages of the peristaltic pump sampling method are it (1) supports a range of sample volumes, (2) reduces sample handling, (3) reduces the potential for contamination, (4) provides flexibility in sampling locations, and (5) supports a variety of filter types. Using stainless-steel mesh filters allows for (1) streamlined and direct field-to-laboratory sample processing, (2) μFTIR transmission mode analysis of filter-mounted microplastics, and (3) reduced filter and sample processing costs.