A study of P release from Fe-P and Ca-P via the organic acids secreted by Aspergillus niger
A study of P release from Fe-P and Ca-P via the organic acids secreted by Aspergillus niger
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DOI：
10.1007/s12275-021-1178-5
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发表时间：
2021-08
影响因子：
3
通讯作者：
D. Tian;Liyan Wang;Jun Hu;Liangliang Zhang;Ningning Zhou;Jingjing Xia;Meiyue Xu;Kianpoor Kalkhajeh Yusef;Shi-mei Wang;Zhen Li;Hongjian Gao
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作者：
D. Tian;Liyan Wang;Jun Hu;Liangliang Zhang;Ningning Zhou;Jingjing Xia;Meiyue Xu;Kianpoor Kalkhajeh Yusef;Shi-mei Wang;Zhen Li;Hongjian Gao
解磷真菌（PSF）已被广泛应用于溶解难溶性磷酸盐（IPs）。然而，PSF通常表现出不同的磷酸盐增溶能力的各种IP。本研究探讨了黑曲霉对磷酸铁的溶解机理（FePO4，Fe-P）和磷酸三钙（Ca3 [PO4] 2，Ca-P）的三羧酸（TCA）循环，黑曲霉具有较高的Ca-P释放磷（P）含量，培养7 d后达到最大值861 mg/L，草酸通过形成草酸钙促进了Ca-P中磷的释放。Fe-P的存在能刺激A.通过柠檬酸合成酶（CS）活性的增强证实了nigerto分泌大量的柠檬酸。而柠檬酸对Fe-P释放的促进作用仅为0.5%。草酸对Fe-P释放的促进作用虽然仍占主导地位，但其丰度明显下降。相反，草酸在Ca-P中也显示出比柠檬酸更高的P释放率，即，36%对22%。本研究指出了A. nigerto溶解IP在土壤中所需的草酸分泌增强。
Phosphate solubilizing fungi (PSF) have been widely applied to dissolve insoluble phosphates (IPs). However, the PSF usually demonstrates a different phosphate solubilizing capacity for various IPs. This study explored the mechanisms ofAspergillus nigerfor the dissolution of ferric phosphate (FePO4, Fe-P), and tricalcium phosphate (Ca3[PO4]2, Ca-P) regarding the tricarboxylic acid (TCA) cycle.Aspergillus nigerhas higher phosphorus (P) content released from Ca-P, reached the maximum value of 861 mg/L after seven days of incubation, compared with the 169 mg/L from Fe-P. Oxalic acid promoted the release of P from Ca-P through the formation of calcium oxalate. The presence of Fe-P can stimulateA. nigerto secrete large amounts of citric acid, confirmed by the enhancement of citrate synthase (CS) activity. However, citric acid only promotes 0.5% of P released from Fe-P. Meanwhile, although oxalic acid still dominates the release of P from Fe-P, its abundance was significantly declined. In contrast, oxalic acid also shows a higher P release ratio in Ca-P than citric acid, i.e., 36%vs.22%. This study points to the future usage ofA. nigerto dissolve IPs in soil required to enhance oxalic acid secretion.