The vital function of Fe3O4@Au nanocomposites for hydrolase biosensor design and its application in detection of methyl parathion.
The vital function of Fe3O4@Au nanocomposites for hydrolase biosensor design and its application in detection of methyl parathion.
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DOI：
10.1039/c2nr33107a
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发表时间：
2013-01
期刊：
影响因子：
6.7
通讯作者：
Yuting Zhao;Weiying Zhang;Yuehe Lin;Dan Du
中科院分区：
文献类型：
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作者：
Yuting Zhao;Weiying Zhang;Yuehe Lin;Dan Du
以半胱胺（SH-NH（2））为连接剂，制备了纳米金修饰磁性Fe 3 O 4核的纳米复合材料，并通过TEM、XPS、UV和电化学等手段对其进行了表征。在此基础上，基于甲基磷离子水解酶（MPH）在Fe（3）O（4）@Au纳米复合材料上的自组装，研制了一种用于有机磷农药（OP）甲基磷离子检测的水解酶生物传感器。磁性纳米复合材料提供了一种简单的方法来构建酶生物传感器，通过简单地施加一个外部磁场，也提供了一种简单的方法来更新电极表面，通过删除磁铁。与基于抑制的酶生物传感器不同，水解酶不会被OP毒害，因此可重复使用以进行连续测量。金纳米粒子不仅提供大的表面积、高负载效率和快速的电子传递，而且通过静电相互作用稳定酶。MPH生物传感器具有快速响应和高选择性，线性范围为0.5 ~ 1000 ng mL（-1），检测限为0.1 ng mL（-1）。它还显示出可接受的重现性和稳定性。该方法简单易行，为农药的现场检测提供了一种很有前途的策略，构建一个强大的生物传感器。
A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe(3)O(4) core was synthesized using cysteamine (SH-NH(2)) as linker, and characterized by TEM, XPS, UV and electrochemistry. Then a hydrolase biosensor, based on self-assembly of methyl parathion hydrolase (MPH) on the Fe(3)O(4)@Au nanocomposite, was developed for sensitive and selective detection of the organophosphorus pesticide (OP) methyl parathion. The magnetic nanocomposite provides an easy way to construct the enzyme biosensor by simply exerting an external magnetic field, and also provides a simple way to renew the electrode surface by removing the magnet. Unlike inhibition-based enzyme biosensors, the hydrolase is not poisoned by OPs and thus is reusable for continuous measurement. AuNPs not only provide a large surface area, high loading efficiency and fast electron transfer, but also stabilize the enzyme through electrostatic interactions. The MPH biosensor shows rapid response and high selectivity for detection of methyl parathion, with a linear range from 0.5 to 1000 ng mL(-1) and a detection limit of 0.1 ng mL(-1). It also shows acceptable reproducibility and stability. The simplicity and ease of operation of the proposed method has great potential for on-site detection of P-S containing pesticides and provides a promising strategy to construct a robust biosensor.