Genetic targeting of the brain renin-angiotensin system in transgenic rats: Impact on stress-induced renin release
Genetic targeting of the brain renin-angiotensin system in transgenic rats: Impact on stress-induced renin release
复制标题
DOI：
10.1111/j.1365-201x.2004.01333.x
复制
发表时间：
2004-08-01
期刊：
影响因子：
--
通讯作者：
Bader, M
中科院分区：
文献类型：
--
作者：
Baltatu, O;Campos, LA;Bader, M
遗传技术的进步，允许组织特异性靶向基因操作允许开发的转基因模型的改变，仅在大脑中的肾素-血管紧张素（RAS）。我们已经使用这样的方法来开发具有RAS [TGR（ASrAOGEN）]的脑特异性改变的转基因大鼠，以阐明脑RAS的致病作用及其在不同病理生理过程中的相关性。TGR（ASrAOGEN）大鼠由于星形胶质细胞AOGEN合成的反义抑制，整个脑中的血管紧张素原（AOGEN）水平降低。在这篇综述中，我们的目的是总结经验，利用TGR（ASrAOGEN）大鼠模型研究脑RAS和目前的新结果提供证据，该系统参与应激诱导的肾素释放。
The advance of genetic technologies to permit tissue-specific targeted gene manipulation allowed the development of transgenic models with alterations of the renin-angiotensin (RAS) solely in the brain. We have used such methodology to develop a transgenic rat with a brain specific alteration of the RAS [TGR(ASrAOGEN)], in order to elucidate a causative role for the brain RAS and its relevance in different pathophysiological processes. The TGR(ASrAOGEN) rats have decreased levels of angiotensinogen (AOGEN) throughout the brain because of an antisense inhibition of the astroglial AOGEN synthesis. In this review we aimed at summarizing the experience obtained from utilizing the TGR(ASrAOGEN) rat model to study the brain RAS and present novel results providing evidence for the involvement of this system in stress-induced renin release.