EPITOPE EXPRESSION ON THE BREAST EPITHELIAL MUCIN
EPITOPE EXPRESSION ON THE BREAST EPITHELIAL MUCIN
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DOI：
10.1007/bf01961243
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发表时间：
1992-01-01
影响因子：
3.8
通讯作者：
LAMPORT, DTA
中科院分区：
文献类型：
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作者：
CERIANI, RL;PETERSON, JA;LAMPORT, DTA
使用针对乳汁和乳腺组织制剂、针对血型决定簇和针对其他非乳腺上皮粘蛋白创建的一组单克隆抗体（MoAb）探索了乳腺上皮粘蛋白上的多个表位表达。由于乳腺上皮粘蛋白现在用于乳腺癌的诊断和治疗模式，并且还因为在乳腺癌组织中预期不同程度的改变或不完全糖基化，所以这里使用的抗原靶标是天然粘蛋白和通过HF治疗引入的去糖基化的连续阶段。部分去糖基化增加了核心肽氨基酸序列的暴露，通常增加了单克隆抗体的结合，而它降低或偶尔增加了血型寡糖的结合。MoAb与其他粘蛋白的交叉反应性与乳腺上皮粘蛋白（BEM）低。一些抗BEM肽单克隆抗体的亲和力结合常数的研究预测了碳水化合物参与其表位结构。BEM上不同表位的鉴定，其可能的表位结构的调查，以及在分子糖基化的不同阶段的MoAb结合的研究导致碳水化合物对其表位的贡献的知识，并加强了理解其在乳腺癌诊断，预后和治疗中的各种可能应用中的性能的能力。
Multiple epitope expression on the breast epithelial mucin was explored using a panel of monoclonal antibodies (MoAbs) created against milk and breast tissue preparations, against blood group determinants, and against other non-breast epithelial mucins. Since the breast epithelial mucin is now used in both diagnostic and therapeutic modalities for breast cancer, and also because altered or incomplete glycosylation in varying degrees is expected in breast carcinoma tissue, the antigenic target used here was the native mucin and sequential stages of deglycosylation introduced to it by HF treatment. Partial deglycosylation increased exposure of core peptide amino acid sequences increasing MoAb binding generally, while it either decreased or occasionally increased binding of blood group oligosaccharides. Cross reactivity of MoAbs to other mucins was low with the breast epithelial mucin (BEM). The study of the affinity binding constants of some of the anti-BEM peptide MoAbs predicted carbohydrate participation in their epitope structure. The identification of different epitopes on the BEM, investigations on their possible epitopic structure, and the study of MoAb binding during different stages of glycosylation of the molecule leads to knowledge on the contribution of carbohydrates to their epitopes and strengthens the ability to understand their performance in their diverse possible applications in breast cancer diagnosis, prognosis, and therapy.