DEFECTIVE INTERLEUKIN-2 AND INTERFERON-GAMMA GENE-EXPRESSION IN RESPONSE TO ANTIGEN IN A SUBGROUP OF PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY
DEFECTIVE INTERLEUKIN-2 AND INTERFERON-GAMMA GENE-EXPRESSION IN RESPONSE TO ANTIGEN IN A SUBGROUP OF PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY
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DOI：
10.1016/0091-6749(93)90178-i
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发表时间：
1993-08-01
影响因子：
14.2
通讯作者：
EIBL, MM
中科院分区：
文献类型：
--
作者：
FISCHER, MB;HAUBER, I;EIBL, MM
Patients with common variable immunodeficiency (CVID) are heterogeneous in the clinical manifestations of the disease and in the underlying mechanisms leading to the immunodeficiency The impairment of B-cell function can be due to an intrinsic defect of the B cells, a deficiency in the function of the antigen-presenting cell, or a dysfunction in the course of T-cell activation. In the present report we have focused our attention on T-lymphocyte activation in three patients with CVID. Numbers of T and B cells, as well as CD4 and CD8 T cell subsets, were within the normal range. The patients' B cells secreted IgM but did not secrete IgG and IgA in response to B-cell stimuli in vitro. Addition of allogeneic T cells was followed by an increase in IgM production but had no effect on the other immunoglobulin isotypes. Examination of T-cell function revealed impaired proliferative response, interleukin-2 (IL-2) and interferon-gamma gene expression and IL-2 release after antigenic stimulation, whereas T-cell proliferation, as well as IL-2 gene expression and release in response to stimulation Wa anti-CD3, were comparable to those of healthy control subjects. Anti-CD3-induced IFN-gamma gene activation in T cells from two patients was comparable to that of control subjects, whereas T cells from the third patient showed reduced expression of IFN-gamma mRNA. In contrast to the decreased IL-2 and IFN-gamma mRNA levels, IL-2R transcripts examined in parallel were normal in CVID T cells on stimulation with antigen. The defect in IL-2 and IFN-gamma mRNA expression after stimulation with antigen, but not with anti-CD3, suggests an abnormality confined to T-cell activation by the T-cell receptor.
