Periodontitis increases vascular cyclooxygenase-2: potential effect on vascular tone
Periodontitis increases vascular cyclooxygenase-2: potential effect on vascular tone
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DOI：
10.1111/jre.12083
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发表时间：
2014-02-01
影响因子：
3.5
通讯作者：
Fernandes, D.
中科院分区：
文献类型：
--
作者：
Mendes, R. T.;Sordi, R.;Fernandes, D.
背景和目的：已证明牙周炎会引起全身炎症，可能损害内皮功能。环加氧酶-2 (COX-2) 是炎症过程中的重要酶，负责产生前列环素。我们假设在牙周炎中，血管 COX-2 表达可能增加，这反过来可能在血管稳态中发挥作用。因此，我们在实验性牙周炎大鼠模型中评估了COX-2抑制的血管效应。 材料和方法：通过在下颌第一磨牙和上颌第二磨牙两侧的子宫颈周围放置棉线来诱导大鼠实验性牙周炎。假手术大鼠在手术后立即除去结扎线。获取肠系膜血管用于研究COX-2表达，并收集血样用于一氧化氮定量。在另一组实验中，动物接受依托考昔（10 mg/kg/d，v.o.）或载体，并评估牙槽骨丢失和心血管参数。 结果：我们观察到从牙周炎动物采集的肠系膜血管中 COX-2 表达增加，同时伴随着一氧化氮含量减少。依托考昔治疗损害了牙周炎大鼠内皮依赖性血压降低。结论：牙周炎增加了血管COX-2的表达，这对于维持该模型中的血管稳态很重要。尽管动物研究存在局限性，但这些发现可能对牙周炎患者使用选择性 COX-2 抑制剂的安全性具有重要意义。
Background and objective: It has been demonstrated that periodontitis induces a systemic inflammation, which may impair endothelial function. Cyclooxygenase-2 (COX-2) is an important enzyme in the inflammatory process and is responsible for prostacyclin production. We hypothesised that in periodontitis, an increase in vascular COX-2 expression may occur, which in turn may have a role in vascular homeostasis. Thus, we evaluated the vascular effects of COX-2 inhibition in an experimental rat model of periodontitis.Material and Methods: Experimental periodontitis was induced in rats by placing a cotton ligature around the cervix of both sides of the mandibular first molars and maxillary second molars. Sham-operated rats had the ligature removed immediately after the procedure. Mesenteric vessels were obtained for the study of COX-2 expression, and blood samples were collected for nitric oxide quantification. In another set of experiments, animals received etoricoxib (10 mg/kg/d, v.o.) or vehicle, and alveolar bone loss and cardiovascular parameters were evaluated.Results: We observed an increase in COX-2 expression in mesenteric vessels harvested from animals with periodontitis, which was accompanied by a reduction in nitric oxide content. Etoricoxib treatment impaired the endothelium-dependent reduction in blood pressure in rats with periodontitis.Conclusion: Periodontitis increases vascular COX-2 expression, which is important in the maintenance of vascular homeostasis in this model. Despite the limitations of an animal study, these findings may have important implications regarding the safety of using selective COX-2 inhibitors in patients with periodontitis.