In vitro and in vivo assessment of Salmonella enterica serovar typhimurium DT104 virulence
In vitro and in vivo assessment of Salmonella enterica serovar typhimurium DT104 virulence
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DOI：
10.1128/iai.69.7.4673-4677.2001
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发表时间：
2001-07-01
影响因子：
3.1
通讯作者：
Libby, SJ
中科院分区：
文献类型：
--
作者：
Allen, CA;Fedorka-Cray, PJ;Libby, SJ
多重耐药肠道沙门氏菌血清型鼠伤寒噬菌体DT 104型已成为全球范围内人类和其他动物感染的广泛原因。在沙门氏菌血清型鼠伤寒沙门氏菌DT 104爆发的临床疾病的严重性导致了该菌株具有增强的毒力的建议。对肠道血清型鼠伤寒沙门氏菌DT 104进行了检测，并与沙门氏菌进行了比较。肠血清型鼠伤寒沙门氏菌ATCC 14028s。这些DT 104分离物在小鼠腹腔巨噬细胞内存活、侵入培养的上皮细胞、抵抗活性氧和氮化合物的抗微生物作用以及在小鼠中引起致死性感染的能力被评估。我们的结果未能证明S.肠道血清型鼠伤寒沙门氏菌DT 104分离株比肠道血清型鼠伤寒沙门氏菌ATCC 14028s毒力更强。
Multidrug-resistant Salmonella enterica serovar Typhimurium phage type DT104 has become a widespread cause of human and ether animal infection worldwide. The severity of clinical illness in S, enterica serovar Typhimurium DT104 outbreaks has led to the suggestion that this strain possesses enhanced virulence, In the present study, in vitro and in vivo virulence-associated phenotypes of several clinical isolates of S. enterica serovar Typhimurium DT104 were examined and compared to S. enterica serovar Typhimurium ATCC 14028s. The ability of these DT104 isolates to survive within murine peritoneal macrophages, invade cultured epithelial cells, resist antimicrobial actions of reactive oxygen and nitrogen compounds, and cause lethal infection in mice were assessed. Our results failed to demonstrate that S. enterica serovar Typhimurium DT104 isolates are more virulent than S, enterica serovar Typhimurium ATCC 14028s.