Clonal organization of proliferating spermatogonial stem cells in adult males of two species of non-human primates, Macaca mulatta and Callithrix jacchus
Clonal organization of proliferating spermatogonial stem cells in adult males of two species of non-human primates, Macaca mulatta and Callithrix jacchus
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DOI：
10.1095/biolreprod.104.033092
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发表时间：
2005-02-01
影响因子：
3.6
通讯作者：
Schlatt, S
中科院分区：
文献类型：
--
作者：
Ehmcke, J;Luetjens, CM;Schlatt, S
本研究检查了两种非人类灵长类动物（狨猴和恒河猴）精原干细胞增殖和分化中克隆形成模式的存在。我们开发了一种新方法来检测整个生精小管中增殖的精原细胞克隆。溴脱氧尿苷和顶体蛋白的双重荧光标记与共聚焦显微镜相结合，可以描述生精上皮周期特定阶段增殖精原细胞的克隆形成和空间排列。石蜡包埋组织的横截面用相同的方法标记。对于这两种猴子，我们证明在生精上皮周期的特定阶段存在不同大小的增殖精原细胞克隆。对恒河猴的详细分析显示，A（苍白）精原细胞在生精上皮周期的 VII 和 IX 阶段增殖，B 精原细胞在 II、IV、VI 和 XII 阶段增殖。在周期的第七和第九阶段，增殖的A（苍白）精原细胞成对或四联体组织。 B，精原细胞表现为四联体或八细胞克隆，B，精原细胞表现为 8 或 16 细胞克隆。我们得出的结论是，恒河猴的精子发生是由A（苍白）精原细胞的二联体或四联体的两次分裂启动的：第一次分裂在第七阶段，之后A（苍白）精原细胞的克隆分离，第二次分裂在第九阶段，产生B、精原细胞的克隆以及A（苍白）精原细胞对和四联体的克隆。 精原细胞补充生精上皮以维持 A 精原细胞群的原始大小。
The present study examines the existence of clonogenic patterns in the proliferation and differentiation of spermatogonial stem cells in two species of non-human primates, the marmoset and the rhesus monkey. We developed a novel approach to detect proliferating spermatogonial clones in whole mounts of seminiferous tubules. Dual fluorescence labeling of bromodeoxyuridine and acrosin in conjunction with confocal microscopy allows the description of the clonogenic and spatial arrangement of proliferating spermatogonia at specific stages of the seminiferous epithelial cycle. Cross-sections of paraffin-embedded tissue were labeled by the same approach. For both monkey species we demonstrate the presence of proliferating spermatogonial clones of variable size at specific stages of the cycle of the seminiferous epithelium. Detailed analysis of the rhesus monkey reveals proliferating A(pale) spermatogonia at stages VII and IX of the cycle of the seminiferous epithelium, and of proliferating B spermatogonia at stages II, IV, VI, and XII. Proliferating A(pale) spermatogonia at stages VII and IX of the cycle are organized in pairs or quadruplets. B, spermatogonia appear as quadruplets or eight-cell clones, and B, spermatogonia as 8- or 16-cell clones. We conclude that spermatogenesis in the rhesus monkey is initiated by two divisions of duplets or quadruplets of A(pale) spermatogonia: a first division at stage VII, after which the clones of A(pale) spermatogonia separate, and a second division at stage IX, which leads to clones of B, spermatogonia as well as pairs and quadruplets of A(pale) spermatogonia replenishing the seminiferous epithelium to maintain the original size of the A spermatogonial population.