Capillary changes in hippocampal CA1 and CA3 areas of the aging rhesus monkey
Capillary changes in hippocampal CA1 and CA3 areas of the aging rhesus monkey
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DOI：
10.1007/s004010000227
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发表时间：
2000-12-01
影响因子：
12.7
通讯作者：
Fuchs, E
中科院分区：
文献类型：
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作者：
Keuker, JIH;Luiten, PGM;Fuchs, E
恒河猴被认为是研究人类衰老的有用动物模型，因为非人类灵长类动物显示出许多在衰老人类中报道的神经生物学改变。伴随正常衰老的认知障碍可能至少部分源于海马体中的毛细血管变化，已知海马体与学习和记忆有关。在非人类灵长类动物海马体中，脑血管的相关影响尚未研究。因此，我们研究了老年非人灵长类动物海马中与年龄相关的微血管变化。我们用电子显微镜观察了14只年龄从1岁到31岁的雄性恒河猴（Macaca mulatta）的CA_1和CA_3区的微血管超微结构。半定量测定了4只年轻（1-6岁）、6只中年（17-24岁）和4只老年（29-31岁）猴的基底膜增厚毛细血管百分比和基底膜中胶原蛋白沉积。年轻受试者中基底膜的畸变很少（毛细血管的28 +/- 6%），并且在恒河猴的衰老过程中发生频率增加（老年动物：毛细血管的71 +/- 5%）。这可能归因于与年龄相关的毛细血管数量增加，显示胶原纤维沉积，而不是基底膜局部增厚。观察到的微血管完整性的变化与在人类中观察到的变化非常相似，支持恒河猴作为人类衰老模型的观点。这些变化缓慢但稳定的进展可能不利于神经元的有效营养供应，因此可能导致正常衰老期间认知功能下降。
The rhesus monkey is considered a useful animal model for studying human aging, because non-human primates show many of the neurobiological alterations that have been reported in aging humans. Cognitive impairment that accompanies normal aging may, at least partially, originate from capillary changes in the hippocampus, known to be involved in learning and memory. Age-related effects on the cerebral capillaries in the nonhuman primate hippocampus have not yet been studied. Therefore, we investigated age-related microvascular changes in the hippocampus of the aged non-human primate. We examined by electron microscopy the microvascular ultrastructure in the CA1 and CA3 areas of 14 male rhesus monkeys (Macaca mulatta), ranging from 1 to 31 years of age. The percentages of capillaries showing basement membrane thickening and deposits of collagen in the basement membrane were determined semiquantitatively in 4 young (1-6 years), 6 middle-aged (17-24 years), and 4 aged (29-31 years) monkeys. Aberrations in the basement membrane are few in young subjects (28 +/- 6% of capillaries), and occur with increasing frequency during the aging process in rhesus monkeys (aged animals: 71 +/- 5% of capillaries). This could be ascribed to an aging-associated increasing number of capillaries showing depositions of collagen fibrils, rather than local thickenings of the basement membrane. The observed changes in microvascular integrity are very similar to those seen in humans, supporting the view of rhesus monkeys as a model for human aging. The slow but steady progression of these changes could be detrimental for an efficient nutrient supply of the neuropil, and might therefore contribute to decreased cognitive functioning during normal aging.