Three PIGGYBACK genes that specifically influence leaf patterning encode ribosomal proteins
Three PIGGYBACK genes that specifically influence leaf patterning encode ribosomal proteins
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DOI：
10.1242/dev.016469
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发表时间：
2008-04-01
期刊：
影响因子：
4.6
通讯作者：
Byrne, Mary E.
中科院分区：
文献类型：
--
作者：
Pinon, Violaine;Etchells, J. Peter;Byrne, Mary E.
叶是从茎顶端分生组织的侧面产生的有限器官，作为极性结构，具有明显的近轴（背侧）和远轴（腹侧）。相反的调控基因之间的相互作用，指定近轴或远轴的命运功能，以维持背腹极性。这个调控网络的一个组成部分是Myb结构域转录因子基因ASYMMETRIC LEAVES 1（AS 1）。AS 1对叶极性的贡献在不同的植物物种中各不相同;然而，在拟南芥中，AS 1突变体在叶极性上只有轻微的缺陷，这表明存在叶图案化的替代途径。在这里，我们描述了三个基因，PIGGYY 1（PGY 1），PGY 2和PGY 3，改变叶图案的情况下，AS 1。在as 1突变体背景下，所有三个pgy突变体都在叶片的近轴侧产生了戏剧性的异位叶片生长。这种叶图案缺陷通过近轴HD-ZIPIII基因REVOLUTA（REV）中的突变而增强，并且通过远轴KANADI基因中的突变而抑制。因此，PGY基因通过与HD-ZIPIII-KANADI途径的遗传相互作用影响叶发育。PGY 1、PGY 2和PGY 3分别编码细胞质大亚基核糖体蛋白L10 a、L9和L5。我们的研究结果表明，在叶背腹图案的翻译的作用，并表明核糖体的调节器的关键图案在植物发育中的事件。
Leaves are determinate organs that arise from the flanks of the shoot apical meristem as polar structures with distinct adaxial (dorsal) and abaxial (ventral) sides. Opposing regulatory interactions between genes specifying adaxial or abaxial fates function to maintain dorsoventral polarity. One component of this regulatory network is the Myb-domain transcription factor gene ASYMMETRIC LEAVES1 (AS1). The contribution of AS1 to leaf polarity varies across different plant species; however, in Arabidopsis, as1 mutants have only mild defects in leaf polarity, suggesting that alternate pathways exist for leaf patterning. Here, we describe three genes, PIGGYBACK1 (PGY1), PGY2 and PGY3, which alter leaf patterning in the absence of AS1. All three pgy mutants develop dramatic ectopic lamina outgrowths on the adaxial side of the leaf in an as1 mutant background. This leaf-patterning defect is enhanced by mutations in the adaxial HD-ZIPIII gene REVOLUTA (REV), and is suppressed by mutations in abaxial KANADI genes. Thus, PGY genes influence leaf development via genetic interactions with the HD-ZIPIII-KANADI pathway. PGY1, PGY2 and PGY3 encode cytoplasmic large subunit ribosomal proteins, L10a, L9 and L5, respectively. Our results suggest a role for translation in leaf dorsoventral patterning and indicate that ribosomes are regulators of key patterning events in plant development.