Optofluidic detection of Zika nucleic acid and protein biomarkers using multimode interference multiplexing
Optofluidic detection of Zika nucleic acid and protein biomarkers using multimode interference multiplexing
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DOI：
10.1364/boe.9.003725
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发表时间：
2018-08-01
影响因子：
3.4
通讯作者：
Schmidt, Holger
中科院分区：
文献类型：
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作者：
Stambaugh, Alexandra;Parks, Joshua W.;Schmidt, Holger
最近的大规模寨卡病毒（ZIKV）爆发表明需要快速和特异性诊断技术。检测生物样品中的ZIKV造成了独特的问题：由于Zika抗体与其他黄病毒的交叉反应性，ZIKV的抗体检测是不充分的，并且ZIKV的核酸和蛋白质生物标志物在感染的不同阶段是可检测的。在这里，我们描述了一种新的光流方法，用于使用多模干涉（MMI）波导并行检测不同的分子生物标志物。我们报告了两种ZIKV生物标志物类型的差异化多重检测，其通过组合几种分析技术使用在两个可见波长下的多点激发，具有超过98%的保真度。(C)根据OSA开放获取出版协议的条款，2018年美国光学学会
The recent massive Zika virus (ZIKV) outbreak illustrates the need for rapid and specific diagnostic techniques. Detecting ZIKV in biological samples poses unique problems: antibody detection of ZIKV is insufficient due to cross-reactivity of Zika antibodies with other flaviviruses, and nucleic acid and protein biomarkers for ZIKV are detectable at different stages of infection. Here, we describe a new optofluidic approach for the parallel detection of different molecular biomarkers using multimode interference (MMI) waveguides. We report differentiated, multiplex detection of both ZIKV biomarker types using multi-spot excitation at two visible wavelengths with over 98% fidelity by combining several analysis techniques. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement