Modeling of supercritical fluid extraction of flavonoids from Calycopteris floribunda leaves
Modeling of supercritical fluid extraction of flavonoids from Calycopteris floribunda leaves
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DOI：
10.2478/s11696-013-0451-4
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发表时间：
2014-03
期刊：
影响因子：
2.2
通讯作者：
Xiong Liu;Dong-liang Yang;Jiajia Liu;K. Xu;Guohui Wu
中科院分区：
文献类型：
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作者：
Xiong Liu;Dong-liang Yang;Jiajia Liu;K. Xu;Guohui Wu
本研究的目的是用CO2和共溶剂超临界流体萃取法(SFE)从花瓣莲叶中提取总黄酮。对从提取物中分离出来的潜在抗癌药物先导化合物Pachypodol进行了考察。以乙醇为萃取剂，采用经典有机溶剂萃取法对高压提取法进行评价。采用高效液相色谱法分析了超临界萃取物和CE萃取物在抗氧化活性和厚朴酚含量方面的差异。研究了超临界流体萃取动力学和总萃取曲线(OEC)的数学模型。根据实验数据对模型进行了评估，结果表明Sovová模型的性能最好。采用中心复合设计法对超临界流体萃取过程进行了优化，其中温度和压力均可调节。超临界流体萃取的最佳条件为：压力30 Mpa，温度35℃。
The aim of this study was to obtain flavonoids extracts from Calycopteris floribunda leaves using supercritical fluid extraction (SFE) with CO2 and a co-solvent. Pachypodol, a potential anticancer drug lead compound separated from the extracts, was examined. Classical organic solvent extraction (CE) with ethanol was performed to evaluate the high pressure method. HPLC analysis was introduced to interpret the differences between SFE and CE extracts in terms of antioxidant activity and the concentration of pachypodol. SFE kinetics and mathematical modeling of the overall extraction curves (OEC) were investigated. Evaluation of the models against experimental data showed that the Sovová model performs the best. The supercritical fluid extraction process was optimized using a central composite design (CCD), where temperature and pressure were adjusted. The optimal conditions of SFE were: pressure of 30 MPa and temperature of 35°C.