Chemical and genetical control of induction of monokaryotic fruiting bodies in Coprinus macrorhizus
Chemical and genetical control of induction of monokaryotic fruiting bodies in Coprinus macrorhizus
复制标题
鸡腿菇单核子实体诱导的化学和遗传控制
DOI：
10.1007/bf00339543
复制
发表时间：
1971
期刊：
影响因子：
--
通讯作者：
T. Ishikawa
中科院分区：
文献类型：
--
作者：
I. Uno;T. Ishikawa
从担子菌子实体和双核菌丝体的无细胞提取液中检测到能在担子菌单核菌丝体中有效诱导子实体的诱果物质(FIS)。担子菌本身和其他几个担子菌种的子实体。FIS的诱导性受遗传和环境因素控制。分离到了不添加FIS而形成单核子实体的构成突变体。这些突变体的子实体和单核菌丝体也含有FIS，FIS对高温、酸、碱解以及几种降解蛋白质和核酸的酶都是稳定的。Sephadex G-25柱层析得到两个FIS组分。其中一种含有蛋白质，另一种似乎具有与腺苷-3‘，5’-单磷酸或腺苷-3‘-单磷酸类似的化学性质，具有诱导单核结实的活性。
Fruiting-inducing substance (FIS), which is effective to induce fruiting bodies in monokaryotic mycelia of a strain of the Basidiomycete,Coprinus macrorhizus, has been detected in cell-free extracts of fruiting bodies and dikaryotic mycelia ofC. macrorhizusitself and fruiting bodies of several other Basidiomycete species. Inducibility by FIS was controlled by genetic and environmental factors. Constitutive mutants which form monokaryotic fruiting bodies without addition of FIS were isolated. Fruiting bodies and monokaryotic mycelia of these mutants also contained FIS.FIS was stable to heat, acid and alkaline hydrolysis, and several enzymes degradating proteins and nucleic acids. Two fractions of FIS were obtained after Sephadex G-25 chromatography. One of them contained protein and the other appears to have similar chemical nature as adenosine-3′, 5′-monophosphate or adenosine-3′-monophosphate which were active to induce monokaryotic fruiting.