Ambient Air Currents Activate Corneal Nerves During Ocular Desiccation in Rats: Simultaneous Recordings of Neural Activity and Corneal Temperature.
Ambient Air Currents Activate Corneal Nerves During Ocular Desiccation in Rats: Simultaneous Recordings of Neural Activity and Corneal Temperature.
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DOI：
10.1167/iovs.18-24604
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发表时间：
2018-08-01
影响因子：
4.4
通讯作者：
Rosenblatt MI
中科院分区：
文献类型：
--
作者：
Hirata H;Dallacasagrande V;Mizerska K;Ivakhnitskaia E;Rosenblatt MI
之前我们发现了两种类型的角膜神经元，我们假设它们在流泪中起重要作用。一种称为低阈值冷敏感加干敏感（LT-CS + DS），另一种称为高阈值冷敏感加干敏感（HT-CS + DS）。本研究探讨了影响这些神经元活动的关键刺激，以阐明可能引发这种眼反射的环境因素。单个角膜神经元的细胞外记录从三叉神经节的眼睛刺激，模仿在日常生活中遇到的环境条件。这些变化包括眼干燥和轻微气流，并在同时监测大鼠眼表温度（OST）时显示。结果表明，神经元稳态活动的变化与稳态活动的变化密切相关：在持续性眼脱水过程中，神经元放电活动呈现出许多突然的小幅度增加LT-CS + DS神经元的这些“尖峰”活动通过诱导约0.2-0.1°C的轻微眼表面冷却的微小气流复制; HT-CS + DS神经元的反应与SS-OST的变化不一致或几乎没有“尖峰”活动的证据。这些结果表明，当我们面对环境时，LT-CS + DS神经元在流泪的传入触发中发挥作用，使角膜暴露在盛行的气流中，使眼表轻微冷却。相比之下，HT-CS + DS神经元可能有助于保护眼睛免受极端干燥，当OST或渗透压的眼泪变得有害时，引起伤害性感受诱发的流泪。
Previously we found two types of corneal neurons that we hypothesized to play an important role in tearing. One type is called low threshold–cold sensitive plus dry sensitive (LT-CS + DS), and the other is termed high threshold–cold sensitive plus dry sensitive (HT-CS + DS). The present study examined critical stimuli influencing the activity of these neurons to elucidate environmental factors that may trigger this ocular reflex. Single corneal neurons were extracellularly recorded from the trigeminal ganglia in response to ocular stimuli that mimic environmental conditions one encounters in daily life. They included an ocular desiccation and slight air currents and were presented while simultaneously monitoring the ocular surface temperatures (OST) in rats. The results showed that the changes in steady state (SS) activity of the neurons closely followed the changes in SS OST: during the sustained ocular desiccation, neural firing displayed numerous small sudden increases in activities (“spiking”); these “spiking” activities of LT-CS + DS neurons were replicated by a minute air current that induced slight ocular surface cooling of approximately 0.2–0.1°C; and the responses of HT-CS + DS neurons showed an inconsistent relationship to the changes in SS OST or exhibited little evidence for “spiking” activities. These results suggest that LT-CS + DS neurons play a role in the afferent trigger of tearing as we face the environment, exposing the cornea to prevailing air currents that produce a slight cooling of the ocular surface. By contrast, HT-CS + DS neurons may serve to protect the eyes from extreme dryness by eliciting nociception-evoked tearing when the OST or osmolarity of tears becomes injurious.
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影响因子：
1.4
作者：
Nosch, Daniela Sonja;Pult, Heiko;Murphy, Paul John
通讯作者：
Murphy, Paul John
影响因子：
5.5
作者：
BELMONTE, C;GALLAR, J;REBOLLO, I
通讯作者：
REBOLLO, I
影响因子：
3.9
作者：
MORGAN, PB;TULLO, AB;EFRON, N
通讯作者：
EFRON, N
影响因子：
3.4
作者：
Acosta, MC;Gallar, J;Belmonte, C
通讯作者：
Belmonte, C
影响因子：
82.9
作者：
Parra, Andres;Madrid, Rodolfo;Belmonte, Carlos
通讯作者：
Belmonte, Carlos