Reduction of inflammatory reaction in the use of purified alginate microcapsules
Reduction of inflammatory reaction in the use of purified alginate microcapsules
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DOI：
10.1080/09205063.2012.735100
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发表时间：
2013-06-01
影响因子：
3.6
通讯作者：
Khang, Gilson
中科院分区：
文献类型：
--
作者：
Kim, A. Ram;Hwang, Ji Hye;Khang, Gilson
藻酸盐，一种从褐藻中提取的多糖，仍然是用于固定待移植细胞的最广泛使用的生物材料，因为包封细胞的良好活力和相对容易的细胞包封加工。然而，主要缺点是体内免疫反应。为了克服这个问题，我们已经证明了一种改进的Korbutt方法用于藻酸盐纯化。海藻酸钠微胶囊制成后，将NIH/3 T3成纤维细胞接种在纯化和未纯化的海藻酸钠微胶囊中，并通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物测定法分析细胞增殖。进行逆转录-聚合酶链反应以评估RAW 264.7巨噬细胞的炎症细胞因子如TNF-α的mRNA表达。将纯化和未纯化的海藻酸钠微囊植入Wister大鼠体内，1- 2周后取出。收获植入物周围的组织，并通过H&E染色进行组织学评价，通过艾德-1染色进行免疫组织化学评价。在该结果中，纯化的藻酸盐中的污染物质通过纯化过程被去除。因此，炎性细胞的密度比未纯化的藻酸盐减少约30%，纤维化壁的厚度减少约三倍。总之，预期纯化的藻酸盐对于许多生物材料应用是高度有效的。
Alginate, a polysaccharide extracted from brown seaweed, remains the most widely used biomaterial for immobilizing cells to be transplanted, because of the good viability of the encapsulated cells and the relatively ease of processing for cell encapsulation. However, the main drawback is the immune reaction in vivo. To overcome this problem, we have demonstrated a modified Korbutt method for alginate purification. After alginate microcapsules were manufactured, NIH/3T3 fibroblast cells were seeded in purified and non-purified alginate microcapsules, and the cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. Reverse transcriptase-polymerase chain reaction was performed to assess the mRNA expression of RAW 264.7 macrophage cells for inflammation cytokines such as TNF-. Purified and non-purified alginate microcapsules were implanted into Wister rats, and subsequently extracted after 1-2weeks. Tissues surrounding the implants were harvested and underwent histological evaluation through H&E staining and immunohistochemical evaluation through ED-1 staining. In this result, contaminated materials in the purified alginate were eliminated by purification process. Thereby, density of inflammatory cell decreased about 30% more than non-purified alginate and thickness of fibrotic wall decreased about three times. In concluding, the purified alginate is anticipated to be highly potent for numerous biomaterial applications.