Mutational analysis of branching in pea.: Evidence that Rms1 and Rms5 regulate the same novel signal
Mutational analysis of branching in pea.: Evidence that Rms1 and Rms5 regulate the same novel signal
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DOI：
10.1104/pp.126.3.1205
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发表时间：
2001-07-01
期刊：
影响因子：
7.4
通讯作者：
Beveridge, CA
中科院分区：
文献类型：
--
作者：
Morris, SE;Turnbull, CGN;Beveridge, CA
此处描述了来自豌豆 (Pisum sativum) 的第五种增加分枝 (rms) 突变体 rms5 的表型和与其他四种 rms 突变体的嫁接反应。 rms5 植物的木质部汁液玉米素核苷浓度和芽生长素水平也与 rms1 和野生型 (WT) 进行了比较。 Rms1和Rms5似乎在生化或细胞水平上密切作用以控制分枝，因为分枝在rms5或rms1与WT植物之间的相互上胚轴嫁接中受到抑制，但在rms5和rmsl幼苗之间的相互嫁接中不受抑制。 rmsl rms5 双突变体的弱海侵或轻微加性表型为这种相互作用提供了进一步的证据。与 rms1 一样，rms5 砧木的木质部汁液细胞分裂素浓度降低，并且 rms5 芽似乎不缺乏吲哚-3-乙酸或 4-氯吲哚-3-乙酸。 Rms1 和 Rms5 与其他 Rms 基因的相互作用相似。 rmsl、rms2 和 rms5 的相互嫁接研究，以及根木质部汁液细胞分裂素浓度在 rms1 和 rms5 中降低而在 rms2 植物中升高的事实，表明 Rms1 和 Rms5 可能控制与 Rms2 控制的途径不同的途径。我们的研究表明，Rms1 和 Rms5 可能调节一种参与分支控制的新型移植物传递信号。
The fifth increased branching ramosus (rms) mutant, rms5, from pea (Pisum sativum), is described here for phenotype and grafting responses with four other rms mutants. Xylem sap zeatin riboside concentration and shoot auxin levels in rms5 plants have also been compared with rms1 and wild type (WT). Rms1 and Rms5 appear to act closely at the biochemical or cellular level to control branching, because branching was inhibited in reciprocal epicotyl grafts between rms5 or rms1 and WT plants, but not inhibited in reciprocal grafts between rms5 and rmsl seedlings. The weakly transgressive or slightly additive phenotype of the rmsl rms5 double mutant provides further evidence for this interaction. Like rms1, rms5 rootstocks have reduced xylem sap cytokinin concentrations, and rms5 shoots do not appear deficient in indole-3-acetic acid or 4-chloroindole-3-acetic acid. Rms1 and Rms5 are similar in their interaction with other Rms genes. Reciprocal grafting studies with rmsl, rms2, and rms5, together with the fact that root xylem sap cytokinin concentrations are reduced in rms1 and rms5 and elevated in rms2 plants, indicates that Rms1 and Rms5 may control a different pathway than that controlled by Rms2. Our studies indicate that Rms1 and Rms5 may regulate a novel graft-transmissible signal involved in the control of branching.