FLAVONE ACETIC-ACID INDUCED CHANGES IN HUMAN ENDOTHELIAL PERMEABILITY - POTENTIATION BY TUMOR-CONDITIONED MEDIUM
FLAVONE ACETIC-ACID INDUCED CHANGES IN HUMAN ENDOTHELIAL PERMEABILITY - POTENTIATION BY TUMOR-CONDITIONED MEDIUM
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DOI：
10.1016/0959-8049(92)90056-8
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发表时间：
1992-01-01
影响因子：
8.4
通讯作者：
WOODCOCK, M
中科院分区：
文献类型：
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作者：
WATTS, ME;WOODCOCK, M
黄酮乙酸（FAA）在小鼠体内系统中引起较大的已建立肿瘤的显著消退。FAA的这种体内作用已被证明包括血管成分。为了阐明FAA的作用机制，我们在体外研究了FAA对人内皮细胞通透性的影响。将在聚碳酸酯滤器上生长的人脐静脉内皮细胞（HUVEC）单层在1 mg/ml FAA中于37 ℃孵育120分钟。在第一个60分钟，有一个6-8倍的渗透性增加，这是随后返回到控制水平，即使在持续存在的FAA。相比之下，在肿瘤条件培养基的存在下，FAA引起渗透性迅速增加6倍，随后没有恢复到对照水平。在后一种条件下发生的渗透性变化伴随着细胞骨架的快速收缩。人黑色素瘤细胞单层的渗透性不受FAA的影响。
Flavone acetic acid (FAA) causes significant regression of larger established tumours in murine in vivo systems. This in vivo effect of FAA has been shown to include a vascular component. In an effort to elucidate the mechanism of action of FAA, we have studied the effects of FAA on the permeability of human endothelium in vitro. Monolayers of human umbilical vein endothelial cells (HUVEC) grown on polycarbonate filters were incubated in 1 mg/ml FAA for 120 min at 37-degrees-C. During the first 60 min, there was a 6-8-fold increase in permeability; this was followed by a return to control levels even in the continued presence of FAA. In contrast, in the presence of tumour conditioned medium, FAA caused a rapid 6-fold increase in permeability which did not subsequently return to control levels. The permeability changes which occurred under the latter conditions were accompanied by a rapid contraction of the cytoskeleton. The permeability of monolayers of human melanoma cells was unaffected by FAA.