Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers.
Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers.
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DOI：
10.1186/s40168-018-0451-2
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发表时间：
2018-04-11
期刊：
影响因子：
15.5
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Dai Z;Coker OO;Nakatsu G;Wu WKK;Zhao L;Chen Z;Chan FKL;Kristiansen K;Sung JJY;Wong SH;Yu J
肠道微生物群的改变与不同人群中的结直肠癌（CRC）相关，并且发现几种细菌物种有助于肿瘤发生。肠道微生物作为早期诊断标志物的潜在用途也有报道。然而，队列特定的噪音可能会扭曲结直肠癌中微生物生态失调的结构，并导致研究结果不一致。在这方面，我们的研究旨在探索在物种水平上跨人群普遍存在的肠道微生物群的变化。基于对来自中国、奥地利、美国、德国和法国队列的526个宏基因组样本的综合分析，在不同人群中鉴定出了7种富含CRC的细菌（脆弱拟杆菌、具核梭杆菌、解砷卟啉单胞菌、微小单胞菌、中间普雷沃氏菌、阿利斯提普斯·菲尼戈迪和嗜酸嗜热厌氧弧菌）。七种富集的细菌标志物将病例与对照进行分类，不同人群的受试者工作特征曲线下面积（AUC）为0.80。相关性分析表明，CRC富集菌和CRC缺失菌分别形成了各自的互惠网络，其中CRC缺失菌在CRC中是脱节的。已发现富含CRC的细菌与脂多糖和能量生物合成途径相关。我们的研究确定了潜在的诊断性细菌标记物，这些标记物在人群中具有很强的鲁棒性，表明它们在非侵入性CRC诊断中具有潜在的普遍用途。我们还阐明了CRC相关微生物群的生态网络和功能能力。本文的在线版本（10.1186/s40168-018-0451-2）包含补充材料，可供授权用户使用。
Alterations of gut microbiota are associated with colorectal cancer (CRC) in different populations and several bacterial species were found to contribute to the tumorigenesis. The potential use of gut microbes as markers for early diagnosis has also been reported. However, cohort specific noises may distort the structure of microbial dysbiosis in CRC and lead to inconsistent results among studies. In this regard, our study targeted at exploring changes in gut microbiota that are universal across populations at species level. Based on the combined analysis of 526 metagenomic samples from Chinese, Austrian, American, and German and French cohorts, seven CRC-enriched bacteria (Bacteroides fragilis, Fusobacterium nucleatum, Porphyromonas asaccharolytica, Parvimonas micra, Prevotella intermedia, Alistipes finegoldii, and Thermanaerovibrio acidaminovorans) have been identified across populations. The seven enriched bacterial markers classified cases from controls with an area under the receiver-operating characteristics curve (AUC) of 0.80 across the different populations. Abundance correlation analysis demonstrated that CRC-enriched and CRC-depleted bacteria respectively formed their own mutualistic networks, in which the latter was disjointed in CRC. The CRC-enriched bacteria have been found to be correlated with lipopolysaccharide and energy biosynthetic pathways. Our study identified potential diagnostic bacterial markers that are robust across populations, indicating their potential universal use for non-invasive CRC diagnosis. We also elucidated the ecological networks and functional capacities of CRC-associated microbiota. The online version of this article (10.1186/s40168-018-0451-2) contains supplementary material, which is available to authorized users.
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人类结直肠癌微生物组的多样化模式
影响因子：
4.2
作者：
Geng J;Fan H;Tang X;Zhai H;Zhang Z
通讯作者：
Zhang Z
影响因子：
7
作者：
Cohn, LD;Becker, BJ
通讯作者：
Becker, BJ
影响因子：
3.7
作者：
Kwok LY;Zhang J;Guo Z;Gesudu Q;Zheng Y;Qiao J;Huo D;Zhang H
通讯作者：
Zhang H
影响因子：
32.4
作者：
Gur, Chamutal;Ibrahim, Yara;Isaacson, Batya;Yamin, Rachel;Abed, Jawad;Gamliel, Moriya;Enk, Jonatan;Bar-On, Yotam;Stanietsky-Kaynan, Noah;Coppenhagen-Glazer, Shunit;Shussman, Noam;Almogy, Gideon;Cuapio, Angelica;Hofer, Erhard;Mevorach, Dror;Tabib, Adi;Ortenberg, Rona;Markel, Gal;Miklic, Karmela;Jonjic, Stipan;Brennan, Caitlin A.;Garrett, Wendy S.;Bachrach, Gilad;Mandelboim, Ofer
通讯作者：
Mandelboim, Ofer
DOI：
10.1214/12-aoas592
发表时间：
2013-03-01
期刊：
The annals of applied statistics
影响因子：
--
作者：
Chen J;Li H
通讯作者：
Li H