Production of biomass and lipid by the microalgae Chlorella protothecoides with heterotrophic-Cu(II) stressed (HCuS) coupling cultivation.
Production of biomass and lipid by the microalgae Chlorella protothecoides with heterotrophic-Cu(II) stressed (HCuS) coupling cultivation.
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DOI：
10.1016/j.biortech.2013.08.153
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发表时间：
2013-11
影响因子：
11.4
通讯作者：
Yuqin Li;J. Mu;Di Chen;Fangxin Han;Hua Xu;Feng Kong;F. Xie;Bo Feng
中科院分区：
文献类型：
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作者：
Yuqin Li;J. Mu;Di Chen;Fangxin Han;Hua Xu;Feng Kong;F. Xie;Bo Feng
本文首次研究了异养Cu（II）胁迫（HCuS）对小球藻细胞脂质积累的影响及其分子基础。结果表明，在此优化策略下，生物量和油脂产量分别达到6.47 g/L和5.78 g/L。脂肪酸组成（几乎100%为C15至C20）是制备高品质生物柴油的理想选择。另外，HCuS诱导的30个差异表达蛋白涉及碳水化合物代谢、碳固定、TCA循环、脂质代谢、蛋白质合成、转运和调控、ATP和RNA合成、核苷酸代谢、ROS清除等。尤其是糖酵解途径可能是脂质积累的重要原因。对这些蛋白质的进一步功能分析将有助于揭示脂质生物合成途径的更多信息。
This work for the first time investigated lipid accumulation by a two-stage regime namely heterotrophic-Cu(II) stressed (HCuS) and underlying molecular basis of lipid biosynthesis inChlorella protothecoidescells. The results showed that the optimized biomass and lipid yield were achieved by 6.47 g/L and 5.78 g/L with this strategy. The fatty acids compositions (almost 100% of them are C15 to C20) are ideal for preparing high quality biodiesel. Further, 30 differentially expressed proteins response to HCuS were involved in carbohydrate metabolism, carbon fixation, TCA cycle, lipid metabolism, protein biosynthesis, transportation and regulation, ATP and RNA biosynthesis, nucleotide metabolism, ROS scavenging. Especially, glycolysis pathway might be the important contributor for lipid accumulation. In future, further functional analysis of these altered proteins would help to reveal more concerning lipid biosynthesis pathway.