Capturing Rare Conductance in Epithelia with Potentiometric-Scanning Ion Conductance Microscopy.
Capturing Rare Conductance in Epithelia with Potentiometric-Scanning Ion Conductance Microscopy.
复制标题
用电位扫描离子电导显微镜捕获上皮细胞中的稀有电导。
DOI：
10.1021/acs.analchem.6b02392
复制
发表时间：
2016
影响因子：
7.4
通讯作者：
Baker,LaneA
中科院分区：
文献类型：
--
作者：
Zhou,Lushan;Gong,Yongfeng;Sunq,Abby;Hou,Jianghui;Baker,LaneA
紧密连接（TJ）是上皮细胞的屏障形成结构，并且可以被描述为紧密密封的细胞间隙。已经广泛地研究了双细胞TJ（bTJ）的传输特性。三细胞连接（tTJ）的屏障功能的知识是不太好理解，主要是由于缺乏适当的技术，以本地测量离散tTJ性能在一个更大的面积的上皮。在这项研究中，我们使用纳米级移液管精确定位tTJ上皮细胞内，并测量tTJ的表观局部电导的技术称为电位扫描离子电导显微镜（P-SICM）。P-SICM显示了区分通过tTJ和bTJ的转运的能力，这在以前的技术和测定中是不可能的。我们描述了野生型和三纤维素过表达的Madin-Darby犬肾（株II，MDCKII）细胞的P-SICM调查。
Tight junctions (TJs) are barrier forming structures of epithelia and can be described as tightly sealed intercellular spaces. Transport properties have been extensively studied for bicellular TJs (bTJs). Knowledge of the barrier functions of tricellular junctions (tTJs) are less well understood, due largely to a lack of proper techniques to locally measure discrete tTJ properties within a much larger area of epithelium. In this study, we use a nanoscale pipet to precisely locate tTJs within epithelia and measure the apparent local conductance of tTJs with a technique termed potentiometric scanning ion conductance microscopy (P-SICM). P-SICM shows the ability to differentiate transport through tTJs and bTJs, which was not possible with previous techniques and assays. We describe P-SICM investigations of both wild type and tricellulin overexpression Madin-Darby Canine Kidney (strain II, MDCKII) cells.