DETECTION OF EPITHELIAL CANCER-CELLS IN PERIPHERAL-BLOOD BY REVERSE-TRANSCRIPTASE POLYMERASE CHAIN-REACTION
DETECTION OF EPITHELIAL CANCER-CELLS IN PERIPHERAL-BLOOD BY REVERSE-TRANSCRIPTASE POLYMERASE CHAIN-REACTION
复制标题
DOI：
10.1038/bjc.1995.56
复制
发表时间：
1995-02-01
影响因子：
8.8
通讯作者：
SELBY, P
中科院分区：
文献类型：
--
作者：
BURCHILL, SA;BRADBURY, MF;SELBY, P
血液中的循环癌细胞在转移过程中起着核心作用。它们的数量可能很少，检测技术必须既敏感又特异。聚合酶链反应（PCR）已成功地用于检测血液系统癌症中的少量肿瘤细胞，其中DNA异常足够一致，使其成为可能。对于大多数实体瘤来说，这还不可行。然而，我们已经发现，逆转录酶（RT）-PCR的组织特异性基因表达是一个有用的技术，用于识别黑色素瘤和神经母细胞瘤患者的少量循环细胞。本文报道了以CK 20 mRNA为标记物的RT-PCR检测结肠癌细胞的方法。与其他检查的细胞角蛋白基因（CK 8和CK 19）不同，CK 20在正常造血细胞中不转录。提示以CK 20为靶基因的RT-PCR在检测结肠癌血液和骨髓转移中的作用。未来的临床资料分析将确定这项技术的临床意义。
Circulating cancer cells in the blood play a central role in the metastatic process. Their numbers can be very small and techniques for their detection need to be both sensitive and specific. Polymerase chain reaction (PCR) has been successfully used to detect small numbers of tumour cells in haematological cancers, in which abnormalities in DNA are sufficiently consistent to make this possible. For most solid tumours this is not yet feasible. However, we have found that reverse transcriptase (RT)-PCR for tissue-specific gene expression is a useful technique for identifying small numbers of circulating cells in melanoma and neuroblastoma patients. In this report we describe detection of colon carcinoma cells by RT-PCR using CK 20 mRNA as a marker. Unlike other cytokeratin genes examined (CK 8 and CK 19), CK 20 was not transcribed in normal haematopoietic cells. This suggests a role for RT-PCR in the detection of colon carcinoma metastasis in blood and bone marrow, using CK 20 as the target gene. Future analysis of clinical material will determine the clinical significance of this technique.