Development of a Microtiter Plate Enzyme-linked Immunosorbent Assay for 17 α, 20 β-21-trihydroxy-4-pregnen-3-one, a Teleost Gondadal Steroid
Development of a Microtiter Plate Enzyme-linked Immunosorbent Assay for 17 α, 20 β-21-trihydroxy-4-pregnen-3-one, a Teleost Gondadal Steroid
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开发用于 17 α, 20 β-21-三羟基-4-pregnen-3-one（一种硬骨鱼性腺类固醇）的微量滴定板酶联免疫吸附测定法
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
T. Higashi
中科院分区：
文献类型：
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作者：
K. Asahina;A. Kambegawa;T. Higashi
以20ƒÀ-S-3-CMO-horseradish过氧化物酶为标记物和兔培养的同源抗血清，建立了一种简单的固相微滴板酶联免疫吸附测定法（ELISA），用于鉴定诱导硬骨鱼成熟的甾体激素- 17 -¿，20ƒÀ， 21-三羟基-4-孕-3-one （20ƒÀ-S）。第二抗体包被到板上，用标记或未标记的20/3-S捕获抗血清。在3小时内，获得了覆盖1.5-192 ƒÊg/井的陡峭标准曲线。该方法的灵敏度和精密度与常规类固醇放射免疫测定法基本相同。这种非同位素分析系统还可以快速测量鲶鱼血浆中的20ƒÀ-S，具有良好的特异性。
A simple solid-phase microtiter plate enzyme-linked immunosorbent assay (ELISA) for 17ƒ¿ , 20ƒÀ, 21-trihydroxy-4-pregnen-3-one (20ƒÀ-S), a teleost maturation inducing steroid, was developed using 20ƒÀ-S-3-CMO-horseradish peroxidase as the label, and a homologous antiserum raised in rabbits. Second antibody coated to the plate was used to capture the antiserum with labeled or unlabeled 20/3-S. Within three hours, a steep standard curve covering 1.5-192 ƒÊg/well was obtained. The sensitivity and precision of this ELISA was almost the same as those of ordinary steroid radioimmunoassays. This non-isotopic assay system also allows prompt measurement of 20ƒÀ-S in catfish plasma with good specificity.