PLASMODIUM-FALCIPARUM OOKINETES MIGRATE INTERCELLULARLY THROUGH ANOPHELES-STEPHENSI MIDGUT EPITHELIUM
PLASMODIUM-FALCIPARUM OOKINETES MIGRATE INTERCELLULARLY THROUGH ANOPHELES-STEPHENSI MIDGUT EPITHELIUM
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DOI：
10.1007/bf00931065
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发表时间：
1989-01-01
影响因子：
2
通讯作者：
MEUWISSEN, JHET
中科院分区：
文献类型：
--
作者：
MEIS, JFGM;POOL, G;MEUWISSEN, JHET
用透射电镜观察了恶性疟原虫和伯氏疟原虫动合子在斯氏按蚊中肠上皮中的迁移。钌红（RR）染色，证实了以前的研究结果：恶性疟原虫动合子采取细胞间途径通过中肠上皮。在同一种蚊子中，啮齿类寄生虫伯氏疟原虫似乎占据了细胞内的位置，正如其他作者先前所建议的那样。伯氏疟原虫或恶性疟原虫的细胞内或细胞间动合子迁移可能分别与感染伯氏疟原虫的蚊子在感染的前2天内的较高死亡率有关。证据表明，卵囊的形成早在动合子的迁移过程中就开始了。在上皮细胞和中肠基膜之间定位后，迅速膨胀的卵囊在血腔表面拉伸后者的上覆层，同时在卵囊和上皮细胞之间产生新的基膜。
The migration of Plasmodium falciparum and P. berghei ookinetes through the midgut epithelium in Anopheles stephensi was studied by transmission electron microscopy. With ruthenium red (RR) staining, the results of previous studies were confirmed: P. falciparum ookinetes take an intercellular route through the midgut epithelium. In the same mosquito species, the rodent parasite P. berghei appeared to take an intracellular position, as previously suggested by other authors. The intra- or intercellular ookinete migration of P. berghei or P. falciparum, respectively, can perhaps be related to the higher mortality of P. berghei-infected mosquitoes within the first 2 days of infection. Evidence is presented that oocyst capsule formation begins as early as during the migration of the ookinete. After localization between the epithelial cells and the midgut basal lamina, the rapidly expanding oocyst stretches the overlying layer of the latter at the haemocoelic surface while a new basal lamina is generated between the oocyst and epithelial cell.