A genetic map of the apospory-region in Brachiaria hybrids : identification of two markers closely associated with the trait
A genetic map of the apospory-region in Brachiaria hybrids : identification of two markers closely associated with the trait
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臂形草杂种无孢子区的遗传图谱：鉴定与性状密切相关的两个标记
DOI：
10.1111/j.1601-5223.1998.00153.x
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发表时间：
2004
期刊：
影响因子：
2.7
通讯作者：
M. Hayward
中科院分区：
文献类型：
--
作者：
S. Pessino;C. Evans;Juan Pablo A. Ortiz;I. Armstead;C. Valle;M. Hayward
本研究的目的是在无融合Brachiaria brizantha和有性Brachiaria ruziziensis杂交群体中鉴定出与控制孢子的基因紧密相关的分子标记。由于玉米5号染色体连锁群先前已经与腕鱼属的无融合位点相关，我们使用25个RFLP克隆详细绘制了玉米5号染色体，以系统地覆盖杂交基因组的所有共合区域。还生成了46个AFLP标记。rz567、rz273和cdo507检测到的三个RFLP标记和两个AFLP标记（PAM52-5和PAM49-13）似乎与载脂蛋白区有关。分离数据与先前报道的数据（对应于RFLP标记umc147， umc72, csu134， csu149和RAPD标记OPC4）一起用于生成该区域的完整图谱。标记PAM52-5和PAM49-13分别位于目标位点两侧1.2 cM和5.7 cM处。该图谱显示与玉米5号染色体和水稻2号染色体区域有密切的同源性。
The objective of this work was to identify molecular markers tightly linked to the gene controlling apospory in a hybrid population derived from a cross between apomictic Brachiaria brizantha and sexual Brachiaria ruziziensis. Since a maize chromosome 5 linkage group had previously been associated with the apomixis locus in Brachiaria we used twenty-five RFLP clones that map in detail maize chromosome 5 to cover systematically all syntenic regions of the hybrid genome. Forty-six AFLP markers were also generated. Three RFLP markers detected by rz567, rz273, and cdo507) and two AFLP markers (PAM52-5 and PAM49-13) appeared to be related to the apo-region. Segregation data, together with previously reported data (corresponding to RFLP markers umc147, umc72, csu134, csu149 and RAPD marker OPC4) were used to generate a complete map of the region. Markers PAM52-5 and PAM49-13 were located respectively at 1.2 cM and 5.7 cM either side of the target locus. The map shows close synteny to regions of maize chromosome 5 and rice chromosome 2.