KP1 - A NEW MONOCLONAL-ANTIBODY THAT DETECTS A MONOCYTE MACROPHAGE ASSOCIATED ANTIGEN IN ROUTINELY PROCESSED TISSUE-SECTIONS
KP1 - A NEW MONOCLONAL-ANTIBODY THAT DETECTS A MONOCYTE MACROPHAGE ASSOCIATED ANTIGEN IN ROUTINELY PROCESSED TISSUE-SECTIONS
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DOI：
10.1136/jcp.42.4.414
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发表时间：
1989-04-01
影响因子：
3.4
通讯作者：
MASON, DY
中科院分区：
文献类型：
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作者：
PULFORD, KAF;RIGNEY, EM;MASON, DY
一种新型单克隆抗体 KP1 是针对人肺巨噬细胞的溶酶体部分产生的，可识别多种组织巨噬细胞（例如库普弗细胞生发中心、脾脏和固有层巨噬细胞）和粒细胞前体中的固定抗性表位。通过对正常和反应性淋巴组织的常规处理样品进行测试，确定了其与单核吞噬细胞谱系细胞的广泛反应性。叉指网状细胞未染色或显示有限的细胞质染色，而朗格汉氏细胞和滤泡树突状网状细胞没有反应。通过免疫沉淀或蛋白质印迹进行测试时，KP1 可识别富含巨噬细胞的人体组织中约 110 千道尔顿的分子（尽管后一种方法还显示出分子量分别为 70 和 40 千道尔顿的两个附加成分）。 KP1 对于研究单核细胞/巨噬细胞系统疾病（包括反应性状态和肿瘤状态（例如真正的组织细胞增殖））具有相当大的价值。
A new monoclonal antibody, KP1, raised against a lysosomal fraction of human lung macrophages, recognises a fixation-resistant epitope in a wide variety of tissue macrophages (such as Kupffer cells germinal centre, splenic, and lamina propria macrophages), and in granulocyte precursors. Its broad reactivity with cells of the mononuclear phagocytic lineage was established by testing on routinely processed samples of normal and reactive lymphoid tissues. Interdigitating reticulum cells were unstained or showed limited cytoplasmic staining while Langerhan''s cells and follicular dendritic reticulum cells were unreactive. KP1 recognises a molecule of about 110 kilodaltons in macrophage-rich human tissue when tested by either immunoprecipitation or Western blotting (although the latter procedure also shows two additional components with molecular weights of 70 and 40 kilodaltons). KP1 should be of considerable value for studying disorders of the monocyte/macrophage system, including both reactive and neoplastic states (such as true histiocytic proliferations).