Evaluation of Extrusion Technique for Nanosizing Liposomes.
Evaluation of Extrusion Technique for Nanosizing Liposomes.
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DOI：
10.3390/pharmaceutics8040036
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发表时间：
2016-12-21
期刊：
影响因子：
5.4
通讯作者：
Yuen KH
中科院分区：
文献类型：
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作者：
Ong SG;Chitneni M;Lee KS;Ming LC;Yuen KH
本研究的目的是研究用于纳米脂质体的不同技术的效率。此外，本研究的目的还在于评估用于纳米级脂质体的挤压技术的工艺参数对所得脂质体的尺寸和尺寸分布的影响。为了比较不同纳米尺寸技术的效率，我们使用了以下技术来实现脂质体的纳米尺寸：挤压、超声、冻融超声(FTS)、超声和均质。挤压技术的效率最高，其次是FTS、超声、超声和均质。本研究中使用的挤出机是使用容易获得且相对便宜的设备制造的。通过改变挤压工艺中的工艺参数，研究它们对挤压脂质体的粒径和分布的影响。结果表明，增大挤压过程的流量可以减小挤出脂质体的尺寸，但对尺寸的均匀性有负面影响。此外，脂质体的大小和分布随着膜孔径的减小而减小。研究发现，当通过孔径为0.2µm及以上的过滤器挤压时，所生成的脂质体小于孔径，而当通过孔径小于0.2µm的过滤器挤压时，所得到的脂质体略大于标称孔径。此外，高于前体脂质体转变温度的挤出温度对挤出脂质体的尺寸和粒径分布没有影响。综上所述，在所评价的所有方法中，挤压技术是可重复性的、有效的。此外，挤压工艺中的工艺参数会影响脂质体的大小和分布。因此，需要对工艺参数进行优化，以获得所需的尺寸范围和均匀性，并可用于各种体内应用。
The aim of the present study was to study the efficiency of different techniques used for nanosizing liposomes. Further, the aim was also to evaluate the effect of process parameters of extrusion techniques used for nanosizing liposomes on the size and size distribution of the resultant liposomes. To compare the efficiency of different nanosizing techniques, the following techniques were used to nanosize the liposomes: extrusion, ultrasonication, freeze-thaw sonication (FTS), sonication and homogenization. The extrusion technique was found to be the most efficient, followed by FTS, ultrasonication, sonication and homogenization. The extruder used in the present study was fabricated using readily available and relatively inexpensive apparatus. Process parameters were varied in extrusion technique to study their effect on the size and size distribution of extruded liposomes. The results obtained indicated that increase in the flow rate of the extrusion process decreased the size of extruded liposomes however the size homogeneity was negatively impacted. Furthermore, the liposome size and distribution was found to decline with decreasing membrane pore size. It was found that by extruding through a filter with a pore size of 0.2 µm and above, the liposomes produced were smaller than the pore size, whereas, when they were extruded through a filter with a pore size of less than 0.2 µm the resultant liposomes were slightly bigger than the nominal pore size. Besides that, increment of extrusion temperature above transition temperature of the pro-liposome had no effect on the size and size distribution of the extruded liposomes. In conclusion, the extrusion technique was reproducible and effective among all the methods evaluated. Furthermore, processing parameters used in extrusion technique would affect the size and size distribution of liposomes. Therefore, the process parameters need to be optimized to obtain a desirable size range and homogeneity, reproducible for various in vivo applications.