Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories
Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories
复制标题
视动盲和易洛魁复合体的互补表达促进折叠形成以分离翼节和铰链区
DOI：
10.1016/j.ydbio.2016.05.020
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发表时间：
2016
影响因子：
2.7
通讯作者：
Shen Jie
中科院分区：
文献类型：
--
作者：
Wang Dan;Li Lingyun;Lu Juan;Liu Suning;Shen Jie
动物形态发生需要褶皱或裂缝来分离细胞群，这些细胞群通常与不同的细胞亲和力有关。在果蝇的成虫盘中，Iroquois复合体（Iro-C）在背中的区域表达导致了铰链/背（H/N）折叠的形成，该折叠将翼铰链和背区域分开。虽然Decapentaplegic（Dpp）信号已被揭示为通过Iro-C向背区的限制而对铰链/背细分至关重要，但H/N边界发展成折叠的机制尚不清楚。在此，我们报道了一个Dpp靶基因，optomotor-blind（omb），介导Dpp信号在Iro-C抑制中的作用。ombis在背铰链侧互补表达，沿着H/N边界沿着邻接Iro-C结构域。在背区表达Iro-C，不依赖于已知的Iro-C调节剂Msh和Stat 92 E。跨越假定的H/N边界的eitheromborIro-C基因的均匀操作通过抑制顶端微管富集而显著抑制H/N折叠的形成。异位锐缘或Iro-C或OMB水平的不连续性足以产生异位褶皱形成。这些结果表明，原子带Iro-C不仅互补表达，而且还协同促进H/N折叠的形成。我们的数据有助于了解Dpp信号是如何在组织细分过程中被解释的。
Animal morphogenesis requires folds or clefts to separate populations of cells which are often associated with different cell affinities. In theDrosophilawing imaginal disc, the regional expression of theIroquois complex(Iro-C) in the notum leads to the formation of the hinge/notum (H/N) fold that separates the wing hinge and notum territories. Although Decapentaplegic (Dpp) signaling has been revealed as essential for the hinge/notum subdivision through the restriction ofIro-Ctoward the notum region, the mechanism by which the H/N border develops into a fold is unknown. Here, we report that a Dpp target gene,optomotor-blind(omb), mediates the role of Dpp signaling inIro-Cinhibition.ombis complementarily expressed on the dorsal hinge side, abutting theIro-Cdomain along the H/N border. Ectopicombexpression inhibitsIro-Cin the notum territory, independent of knownIro-Cregulators Msh and Stat92E. Uniform manipulation of eitheromborIro-Cgenes spanning the presumptive H/N border significantly suppresses H/N fold formation via inhibition of the apical microtubule enrichment. Ectopically sharp border or discontinuity in level of Iro-C or Omb is enough to generate ectopic fold formation. These results reveal thatombandIro-Cnot only are complementarily expressed but also cooperate to promote H/N fold formation. Our data help to understand how Dpp signaling is interpreted region-specifically during tissue subdivision.