Preparation of Biocompatible Liquid Marbles Stabilized by Food-Grade Stearate Microparticle for Aerobic Bacteria Cultivation
Preparation of Biocompatible Liquid Marbles Stabilized by Food-Grade Stearate Microparticle for Aerobic Bacteria Cultivation
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DOI：
10.1007/s12010-020-03299-6
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发表时间：
2020-03
影响因子：
3
通讯作者：
Shoma Tanaka;Hiroaki Okano;N. Matsuda;J. Sawai;K. Naoe;M. Imai
中科院分区：
文献类型：
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作者：
Shoma Tanaka;Hiroaki Okano;N. Matsuda;J. Sawai;K. Naoe;M. Imai
液体大理石(Liquid Marble，简称LM)是一种包覆有微米或纳米级颗粒的不粘液滴，具有广阔的应用前景。LMS具有气体或水蒸气可以通过其颗粒壳层自由传输的优点，因此使其成为一种理想的包含好氧微生物的微生物反应器。本研究以食品级硬脂酸钙微粒为稳定剂，成功制备了更安全、更具生物相容性的LMS。随着芯液体积的增加，液芯高度逐渐增大，并达到一个恒定值，这与常规液芯液膜的变化趋势相似。LMS的干燥速率曲线证实了LMS的干燥规律与典型湿粉的干燥规律相似。干燥速度取决于岩心溶液中的盐类和环境湿度。例如，在氯化镁的情况下，通过将湿度从40%RH改变到80%RH，LMS的寿命(LM完全干燥的时间)增加到大约900分钟。这几乎是无盐和相对湿度为40%的情况下的三倍。模型好氧细菌在24小时的培养过程中，枯草芽孢杆菌在LM内活跃地增殖。与富氧静止培养和摇瓶培养相比，在LM培养体系中的增殖能力高于试管培养。综上所述，我们证明了硬脂酸钙LM系统将是一种更安全、更容易获得的含有好氧细菌的微生物反应器的理想候选者。
Liquid marble (LM), a non-stick drop coated with micro- or nano-scale particles, has great potential in a wide range of applications. LMs have an advantageous feature in which gas or vapor can freely transport through their particle shell; therefore, it makes them an ideal candidate to be utilized as microbioreactor containing aerobic microorganisms. In this study, safer and more biocompatible LMs were successfully prepared using a food-grade calcium stearate microparticle as a stabilizer. As the volume of core liquid increased, the height of LM increased and reached a constant value, as a similar trend has been reported in conventional LMs. The drying rate curve of the LMs confirmed that the LMs have a similar pattern with the drying of typical wet powders. The drying rate depended on the salt species in the core solution and the environmental humidity. For instance, in the case of MgCl2, by changing humidity from 40 to 80% RH, the lifetime of LMs (time in which the LM dried completely) was increased to about 900 min. This is nearly three times longer than those have no salt and at 40% RH. Model aerobic bacteriaBacillus subtilishas actively proliferated inside the LM during 24-h incubation. Comparing with the test tube cultivations under O2-rich stationary or O2rich–shaken conditions, the cultivation in the LM system showed a higher proliferation than the test tube systems. As a conclusion, we demonstrated that the calcium stearate LM system would be an ideal candidate for safer and easily available microbioreactor containing aerobic bacteria.