Quantitative dispersion microscopy.
Quantitative dispersion microscopy.
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DOI：
10.1364/boe.1.000347
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发表时间：
2010-09-01
影响因子：
3.4
通讯作者：
Feld M
中科院分区：
文献类型：
--
作者：
Fu D;Choi W;Sung Y;Yaqoob Z;Dasari RR;Feld M
折射率色散是生物成像研究中的一种固有光学性质和有用的对比度来源。在这份报告中，我们提出了第一个分散相成像的活真核细胞。基于定量相位显微术的原理，我们研制了定量色散显微术。双波长定量相位显微镜在310 nm和400 nm波长下进行相位测量，以量化活细胞的色散（折射率增量比）。发现活HeLa细胞的测量色散为1.088左右，这与使用全内反射直接测量蛋白质溶液的色散一致。这种技术，连同定量相显微镜提供的干质量和形态测量，可以证明是一个有用的工具，用于区分不同类型的生物材料和研究生物样品的空间不均匀性。
Refractive index dispersion is an intrinsic optical property and a useful source of contrast in biological imaging studies. In this report, we present the first dispersion phase imaging of living eukaryotic cells. We have developed quantitative dispersion microscopy based on the principle of quantitative phase microscopy. The dual-wavelength quantitative phase microscope makes phase measurements at 310 nm and 400 nm wavelengths to quantify dispersion (refractive index increment ratio) of live cells. The measured dispersion of living HeLa cells is found to be around 1.088, which agrees well with that measured directly for protein solutions using total internal reflection. This technique, together with the dry mass and morphology measurements provided by quantitative phase microscopy, could prove to be a useful tool for distinguishing different types of biomaterials and studying spatial inhomogeneities of biological samples.