Identification of a novel human ankyrin-repeated protein homologous to CARP
Identification of a novel human ankyrin-repeated protein homologous to CARP
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DOI：
10.1006/bbrc.2001.5216
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发表时间：
2001-07-20
影响因子：
3.1
通讯作者：
Ito, H
中科院分区：
文献类型：
--
作者：
Moriyama, M;Tsukamoto, Y;Ito, H
我们克隆了一个新的锚蛋白重复蛋白，Arpp，通过免疫筛选的cDNA文库构建从人食管癌细胞系，TE 1，与针对一个假设的蛋白质编码的反义p53 mRNA的抗体。Arpp蛋白由333个氨基酸组成，在蛋白质的中间部分含有4个锚定样重复基序，在N-末端区域含有PEST样序列和类似于核定位信号的富含赖氨酸的序列，在C-末端区域含有含有共有磷酸化位点的富含脯氨酸的区域。蛋白质序列分析表明，Arpp是同源的（52.7%的同一性）鲤鱼，这是表明参与调控的心脏心室肌球蛋白轻链2基因的转录。正常骨骼肌和心肌中均有Arpp mRNA表达。有趣的是，在双侧心室中可检测到Arpp表达，但在双侧心房和大血管中检测不到，这表明Arpp可能在心室以及骨骼肌中发挥特定功能。(C)北京：科学出版社.
We cloned a novel ankyrin repeat protein, Arpp, by immunoscreening a cDNA library constructed from a human esophageal carcinoma cell line, TE1, with an antibody directed to a hypothetical protein encoded by antisense p53 mRNA. Arpp protein is composed of 333 amino acids and contains four ankyrin-like repeat motifs in the middle portion of the protein, a PEST-like sequence and a lysine-rich sequence similar to a nuclear localization signal in the N-terminal region, and a proline-rich region containing consensus phosphorylation sites in the C-terminal region. Protein sequence analysis revealed that Arpp is homologous (52.7% identity) to Carp which is shown to be involved in the regulation of the transcription of the cardiac ventricular myosin light chain 2 gene. Arpp mRNA was found to be expressed in normal skeletal and cardiac muscle. Interestingly, Arpp expression was detectable in bilateral ventricles, but undetectable in bilateral atria and large vessels, suggesting that Arpp may play a specific function in cardiac ventricles as well as skeletal muscles. (C) 2001 Academic Press.