Nitrosative protein tyrosine modifications: biochemistry and functional significance.
Nitrosative protein tyrosine modifications: biochemistry and functional significance.
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DOI：
10.5483/bmbrep.2008.41.3.194
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发表时间：
2008-03
期刊：
影响因子：
3.8
通讯作者：
Woon-Seok Yeo;Soo Jae Lee;Jung Rok Lee;K. P. Kim
中科院分区：
文献类型：
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作者：
Woon-Seok Yeo;Soo Jae Lee;Jung Rok Lee;K. P. Kim
Nitrosative modifications regulate cellular signal transduction and pathogenesis of inflammatory responses and neurodegenerative diseases. Protein tyrosine nitration is a biomarker of oxidative stress and also influences protein structure and function. Recent advances in mass spectrometry have made it possible to identify modified proteins and specific modified amino acid residues. For analysis of nitrated peptides with low yields or only a subset of peptides, affinity 'tags' can be bait for 'fishing out' target analytes from complex mixtures. These tagged peptides are then extracted to a solid phase, followed by mass analysis. In this review, we focus on protein tyrosine modifications caused by nitrosative stresses and proteomic methods for selective enrichment and identification of nitrosative protein modifications.
