A LARGE ARRAY OF HUMAN MONOCLONAL-ANTIBODIES TO TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS FROM COMBINATORIAL LIBRARIES OF ASYMPTOMATIC SEROPOSITIVE INDIVIDUALS
A LARGE ARRAY OF HUMAN MONOCLONAL-ANTIBODIES TO TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS FROM COMBINATORIAL LIBRARIES OF ASYMPTOMATIC SEROPOSITIVE INDIVIDUALS
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DOI：
10.1073/pnas.88.22.10134
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发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
LERNER, RA
中科院分区：
文献类型：
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作者：
BURTON, DR;BARBAS, CF;LERNER, RA
通过从丝状噬菌体表面表达的随机组合文库中选择抗原，针对 1 型人类免疫缺陷病毒 (HIV) 的表面糖蛋白 gp120 生成了一组人单克隆抗体 Fab 片段。该文库是从一名 HIV 呈阳性 6 年的无症状个体的 5 毫升骨髓中制备的。这些抗体对抗原具有高亲和力（大多数亲和常数> 10(8) M-1）和显着的序列多样性。如果选择适当的供体，所描述的方法应该能够产生抗体，用于评估被动免疫作为艾滋病治疗的效果。
A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of > 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.