Toxicity and deficiency of copper in Elsholtzia splendens affect photosynthesis biophysics, pigments and metal accumulation.
Toxicity and deficiency of copper in Elsholtzia splendens affect photosynthesis biophysics, pigments and metal accumulation.
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DOI：
10.1021/es3050746
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发表时间：
2013-06
影响因子：
11.4
通讯作者：
Hongyun Peng;P. Kroneck;H. Küpper
中科院分区：
文献类型：
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作者：
Hongyun Peng;P. Kroneck;H. Küpper
海州香薷是生长在我国铜矿床上的一种耐铜植物。利用光谱和空间分辨动力学方法研究了在活体条件下，E.以一串红为试材，从铜缺乏、铜毒害以及铜胁迫的适应性等方面研究了铜胁迫对一串红的影响。将植物在含有Fe（III）-EDTA或Fe（III）-EDDHA的营养液中培养。铜毒性对PSIIRC光适应电子流的影响远大于非光化学猝灭（NPQ）或暗适应光化学效率（Fv/Fm）。它还改变了光谱分辨叶绿素荧光动力学，特别是通过加强NPQ的短波长（<700 nm）部分，改变捕光复合物II（LHCII）聚集。铜毒害降低了叶片中铁的积累，降低了叶绿素和类胡萝卜素的含量。在铜毒害的驯化过程中，叶片铜含量下降，铁含量上升，光合活性和色素恢复到正常水平。E.驯化可能与E. plendens体内铜和铁的稳态有关。灿烂。当铜含量为10 mg/kg时，植株生长缓慢，光合参数（F0、Fv/Fm、ΦPSII）下降。这些结果的重要性进行了讨论，以评估植物修复植物在其环境中的压力的反应。
Elsholtzia splendens is a copper-tolerant plant species growing on copper deposits in China. Spatially and spectrally resolved kinetics of in vivo absorbance and chlorophyll fluorescence in mesophyll of E. splendens were used to investigate the copper-induced stress from deficiency and toxicity as well as the acclimation to excess copper stress. The plants were cultivated in nutrient solutions containing either Fe(III)-EDTA or Fe(III)-EDDHA. Copper toxicity affected light-acclimated electron flow much stronger than nonphotochemical quenching (NPQ) or dark-acclimated photochemical efficiency of PSIIRC (Fv/Fm). It also changed spectrally resolved Chl fluorescence kinetics, in particular by strengthening the short-wavelength (<700 nm) part of NPQ altering light harvesting complex II (LHCII) aggregation. Copper toxicity reduced iron accumulation, decreased Chls and carotenoids in leaves. During acclimation to copper toxicity, leaf copper decreased but leaf iron increased, with photosynthetic activity and pigments recovering to normal levels. Copper tolerance in E. splendens was inducible; acclimation seems be related to homeostasis of copper and iron in E. splendens. Copper deficiency appeared at 10 mg copper per kg leaf DW, leading to reduced growth and decreased photosynthetic parameters (F0, Fv/Fm, ΦPSII). The importance of these results for evaluating responses of phytoremediation plants to stress in their environment is discussed.