Apoptosis pattern and alterations of expression of apoptosis-related factors of supporting cells in Kölliker's organ in vivo in early stage after birth in rats.
Apoptosis pattern and alterations of expression of apoptosis-related factors of supporting cells in Kölliker's organ in vivo in early stage after birth in rats.
复制标题
大鼠出生后早期体内科利克器官支持细胞凋亡模式及凋亡相关因子表达变化
DOI：
10.4081/ejh.2017.2706
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发表时间：
2017-08-07
期刊：
影响因子：
--
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Liu J;Cai L;He Y;Yang J
柯氏器是耳蜗发育过程中一个暂时但不可缺少的结构。其内的支持细胞（SC）释放三磷酸腺苷（ATP），这可能在听力开始之前的耳蜗发育中起关键作用。为了揭示新生大鼠Kolliker's器官的凋亡，我们研究了出生后早期发育过程中形态学变化和凋亡相关因子的表达。结果发现，耳蜗Kolliker's器中的SCs数量减少，形态发生沿着耳蜗顶部到底部的梯度变化;耳蜗Kolliker's器中caspase-3、caspase-8、caspase- 9和bcl-2的表达沿着生长发育过程呈波动性变化，在生后3d表达达到高峰。本研究表明，在出生后耳蜗发育过程中，Kolliker氏器的退化具有时间依赖性，这可能是由内源性因素引发的。
Kolliker’s organ is a temporary but indispensable structure in the development of the cochlea. Supporting cells (SCs) within it release adenosine 5’-triphosphate (ATP), which may play a crucial role in cochlear development before the onset of hearing. To reveal the apoptosis of Kolliker’s organ in new-born rats, we studied the morphological changes and expression of apoptosis-related factors during early postnatal development. We found SCs in Kolliker’s organ decreased in number and changed in appearance along the cochlea apex-to-base gradient, and the expression of caspase-3, caspase-8, caspase- 9 and bcl-2 in Kolliker’s organ of the cochlea fluctuated along the course of postnatal development, with an expression peak at postnatal day 3. This study demonstrates a time-dependent degeneration of Kolliker’s organ during postnatal cochlea development, which might be triggered by endogenous factors.