A second-generation YAC contig map of human chromosome 12.
A second-generation YAC contig map of human chromosome 12.
复制标题
人类 12 号染色体的第二代 YAC 重叠群图谱。
作者：
Kenneth S. Krauter;Kate Montgomery;Yoon Sj;Janine M. LeBlanc;Beatrice Renault;I. Marondel;Herdman;Lisa Cupelli;A. Banks;Jonathan Lieman
人类12号染色体约占人类基因组的4.5%，估计有1.35亿个碱基对(Mb)。我们已经开始构建高分辨率的12号染色体物理图谱作为重叠酵母人工染色体(YACs)，以该染色体的第一代物理图谱为基础，覆盖近102Mb的DNA，包括426个高度多态、单态和基于基因的标记。我们还用细胞遗传学方法定位了119个YAC，其中大部分是物理图谱的一部分。因此，该图谱集成了遗传、物理和细胞遗传学数据，并提供了有关该染色体组织的信息，将有助于疾病相关基因的定位和克隆。本文所采用的12号染色体图谱构建策略可用于快速构建人类其他染色体的物理图谱和表达图谱。
Human chromosome 12 constitutes approximately 4.5% of the human genome and has an estimated size of 135 million base pairs (Mb). We have started to construct a high-resolution physical map of chromosome 12 as overlapping yeast artificial chromosomes (YACs), using as a foundation the first-generation physical map of this chromosome covers nearly 102 Mb of DNA and includes 426 highly polymorphic, monomorphic and gene-based markers. We also mapped 119 of the YACs, most of which are part of the physical map, by cytogenetic methods. Thus the map integrates genetic, physical and cytogenetic data and provides information about the organization of this chromosome and will help in the localization and cloning of disease-related genes. The strategy used here to generate the chromosome-12 map could be applied for the rapid construction of physical and expression maps for other human chromosomes.